| 光谱学与光谱分析 |
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| Excitation-Emission Fluorescence Characterization Study of the Three Phenolic Compounds |
| WANG Huan-bo, ZHANG Yu-jun*, XIAO Xue, JIN Dan, ZHAO Nan-jing, YIN Gao-fang, GUO Li-quan, LIU Wen-qing |
| Key Laboratory of Environmental Optics & Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China |
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Abstract The fluorescence intensity information was collected by scanning its fluorescence spectra at different excitation wavelengths. Based on its high sensitivity and selectivity, excitation-emission fluorescence can be widely used for detection of pollutants in the environment. The characterizations of the three phenolic compounds were investigated by this method, and the fluorescence peaks of phenol, m-cresol and thymol were confirmed at 272/300 nm, 274/300 nm and 276/304 nm when the excitation and emission wavelength were set in the range of 240-360 nm and 260-500 nm respectively. The excitation and emission spectra of the three phenolic compounds are very similar because of their analogical structure. The intensity of the spectrum has a good linear relationship with the concentration when the solution concentration is between 0.02 and 1.0 mg·L-1, and the limits of detection can reach at 1 μg·L-1. Results show that the three compounds can be analyzed qualitatively and quantitatively by excitation-emission fluorescence.
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Received: 2009-06-02
Accepted: 2009-09-06
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Corresponding Authors:
ZHANG Yu-jun
E-mail: yjzhang@aiofm.ac.cn
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[1] Fan Yi, Feng Yu-Qi. Anal. Chim. Acta, 2003, 484: 145.
[2] Oscar Ballesteros, Rosario Blanc. Talanta, 2000, 52: 845.
[3] Gatti R, Gioia M G. Anal. Chim. Acta, 2001, 447: 89.
[4] Pavel Kuban, Hugh Flowers. Anal. Chim. Acta, 2001, 437: 115.
[5] Saitoh T, Nakayama Y. J. Chromatogr. A, 2002, 972: 205.
[6] LIAO Fang, YANG Zhen-de, HUANG Qing-hua(廖 芳, 杨振德, 黄庆华). Herald of Medicine(医药导报), 2005, 24: 868.
[7] Drinking Water Regular Indices(生活饮用水常规指标). GB5749—85, Health Standards of the Drinking Water(生活饮用水卫生标准).
[8] FENG Li-juan, XIN Chang-bo(冯立娟, 辛长波). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2002, 19: 267.
[9] Chen Xin-miao, Zhang Tao-zhi. Microchim. Acta, 2006, 155: 415.
[10] Lee Xiao-Pen, Kumazawa Takeshi. Anal. Lett., 2002, 35: 2093.
[11] Llompart M, Plorenzo R A. Analyst, 1997, 122: 133.
[12] Kovacs A, Kende A. J. Chromatogr. A, 2008, 1194: 139.
[13] Fustinoni S, Mercadante R. Journal of Chromatography B, 2005, 817: 309.
[14] Asan A, Isildak I. J. Chromatogr. A, 2003, 988: 145.
[15] King R A, May B L. Analytical Biochemistry, 2009, 384: 27.
[16] Rogers K, R Becker J Y. Field Analytical Chemistry and Technology, 1999, 3: 161.
[17] Tsuruta Y, Watanabe S. Analytical Biochemistry, 1996, 243: 86.
[18] ZHAO De-feng, GAO Xin-qin(赵德丰, 高欣钦). Dyestuff Industry(染料工业), 1995, 32: 1.
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