光谱学与光谱分析 |
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Hydrogen-Bond State Analysis of CellobiohydrolaseⅡ Molecule from Trichoderma Viride |
YU Duo-wei,YUAN Sheng |
Life Science College,Nanjing Normal University, Nanjing 210097,China |
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Abstract Hydrogen-bond states on cellobiohydrolase Ⅱ (CBHⅡ) of trichoderma viride were analysed by laser Raman spectroscopic method used to reveal molecular normal modes of vibration character. The results indicated that hydrogen-bond ability of carbonyl oxygen-atom of amideⅠ was raised in two types of aqueous solution samples(6.0 and 8.0 of pH value) compared to the solid sample. The variation trend of β structure modes was similar between amideⅡ and amideⅠ. As far as the ability of hydrogen-bond forming is concerned, tryptophan (Trp) and tyrosine (Typ) residues are strong hydrogen proton donor in pH 6.0 and solid sample,thus proving the results of previously spatial structure analysis. By analysis of free S—H character,the —S—S— construction in trichoderma viride mature paptide has been proved to be the same as that of trichoderma reesei.
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Received: 2003-09-23
Accepted: 2004-04-08
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Corresponding Authors:
YU Duo-wei
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Cite this article: |
YU Duo-wei,YUAN Sheng. Hydrogen-Bond State Analysis of CellobiohydrolaseⅡ Molecule from Trichoderma Viride [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(04): 544-547.
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URL: |
https://www.gpxygpfx.com/EN/Y2005/V25/I04/544 |
[1] WANG Jian-rong, ZHANG Man-fu, HUANG Tao(王建荣,张曼夫,黄 涛). Acta Genetica Sinica(遗传学报),1995,22(1):74. [2] GAO Pei-ji(高培基). Progress in Natural Science(自然科学进展),2003,13(1):21. [3] Rouvinen J,Bergfors T,Teeri T et al. Science,1990,249:380. [4] SUN Dong-ping, PANG Yan-jun, LI Zhao-lan(孙东平,庞延军,李兆兰). Journal of Xuzhou Normal University(徐州师范学院学报),1996,14(2):62. [5] HUO Hong,WANG Xing-fu,JU Xun,HUANG Wei-dong(霍 红,王幸福,车 迅,黄威东). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2001,21(5):614. [6] Wen Z Q,Thomas Jr G J. Biochemistry,2000,39(1):146. [7] YAN Bo-xu,QI Fei, ZHANG Ying-shu,GAO Pei-ji(阎伯旭,齐 飞,张颖舒,高培基). Progress in Biochemistry and Biophysics(生物化学与生物物理学进展),1999,26(3):233. [8] Benevides J M,Chan G,Lu X J et al. Biochemistry,2000,39(3):537. [9] Arp Z,Autrey D,Laane J,Overman S,Thomas Jr G J. Biochemistry,2001,40(8):2522. [10] Thomas Jr G. J. Annu. Rev. Biophys. Biomol. Struct.,1999, 28: 1.
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