光谱学与光谱分析 |
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Fluorescence Spectroscopy Study of Bovine Serum Albumin Quenched by Metal Ion |
CHEN Xiao-bo,LI Song |
Applied Optics Beijing Area Major Laboratory, Analytic and Testing Center, Beijing Normal University, Beijing 100875, China |
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Abstract The fluorescence spectrum of bovine serum albumin (BSA) influenced by copper ion, sodium ion and molybdenum ion is studied. The copper ion leads to obvious statistic quenching of BSA fluorescence. The dissociation constant of copper ion from BSA is about 2.38×10-4 mol·L-1. NaCl does not have quenching action on BSA fluorescence. It was illustrated that physiological saline, acting as the body fluid of organism, has very good protection action for life. The molybdenum ion has small statistic quenching effect on BSA fluorescence when its concentration is small. However, a new kind of avalanche quenching phenomenon occurs when the concentration of molybdenum ion is about 8.4×10-3 mol·L-1.
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Received: 2004-01-06
Accepted: 2004-03-07
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Corresponding Authors:
CHEN Xiao-bo
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Cite this article: |
CHEN Xiao-bo,LI Song. Fluorescence Spectroscopy Study of Bovine Serum Albumin Quenched by Metal Ion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(02): 309-312.
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URL: |
https://www.gpxygpfx.com/EN/Y2006/V26/I02/309 |
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