Study on the Reaction Mechanism of Chloroacetanilide Herbicides with Urease Using Fluorescence Spectrum and High-Performance Liquid Chromatography
LIU Hui-jun1，ZHAN Xiu-ming1，LI Ke-bin2，LIU Wei-ping1*
1. Institute of Environmental Science, Zhejiang University, Hangzhou 310027, China 2. Department of Chemistry, Northwest University, Xi′an 710069, China
Abstract：The relationship between excess thermodynamic function and binding to urease of four chloroacetanilide herbicides was studied using high-performance liquid chromatography and fluorescence spectrum. The linear relationship between the composition of mobile phase of RP-HPLC and the capacity factor of chloroacetanilide herbicides has been obtained. The excess thermodynamic enthalpy (Δ*) of acetochlor, pretilachlor, butachlor and metolachlor was determined, and the binding constant K and the number of binding sites with urease were calculated. The relationship between excess thermodynamic function and the binding constant K was suggested.
刘惠君1，詹秀明1，李克斌2，刘维屏1* . 光谱法和色谱法研究酰胺类除草剂与脲酶的结合作用[J]. 光谱学与光谱分析, 2005, 25(03): 463-466.
LIU Hui-jun1，ZHAN Xiu-ming1，LI Ke-bin2，LIU Wei-ping1* . Study on the Reaction Mechanism of Chloroacetanilide Herbicides with Urease Using Fluorescence Spectrum and High-Performance Liquid Chromatography . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 463-466.
