Abstract：The binding of metolachlor and S-Isomer to urease in aqueous solution was studied using UV difference spectrum and fluorescence spectrum. According to the results of the UV difference spectrum curve, the UV difference adsorption ΔA changed rapidly when herbicide concentration increased from 0.0 to 1.6 μmol·L-1. UV difference spectra were red-shifted and absorb-peaks became lower. It was shown that herbicide can quench the urease fluorescence mainly through a static quenching procedure. The binding constant K and the number of binding site n were calculated according to the fluorescence quenching results. For metolachlor, K＝1.49×103 L·mol-1 and n was 0.84; for S-isomer, K＝2.22×103 L·mol-1 and n was 0.89.
刘惠君，刘维屏，陈爱平，杨炜春 . 光谱法研究异丙甲草胺及其S-对映体与脲酶的相互作用机制 [J]. 光谱学与光谱分析, 2004, 24(02): 166-168.
LIU Hui-jun, LIU Wei-ping, CHEN Ai-ping, YANG Wei-chun . The Reaction Mechanism of Metolachlor and S-Isomer with Urease . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(02): 166-168.
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