Abstract：A cationic dye pentamethyl-p-rosaniline hydrochloride 6B was used as a chromic probe and its binding reaction with nucleic acids was studied. In the weak alkalescent medium, the cationic dye showed a decrease in absorption at its maximal absorption peak (584.5 nm) on binding to nucleic acids. Based on this fact, a new UV-Vis spectrophotometric method for the determination of nucleic acids has been developed. Effects of ion-intensity, surfactant and organic solvent were investigated to establish optimum reaction conditions, and then nucleic acids were detected with quantitative analysis. The calibration curves of nucleic acids such as hs-DNA, sm-DNA, ct-DNA and yeast-RNA were linear over the ranges 0.50-4.00 μg·mL-1, 0.20-5.00 μg·mL-1, 0.20-5.00 μg·mL-1 and 0.20-4.50 μg·mL-1, respectively. The detection limits (3σ/ K) were 0.082, 0.037, 0.038 and 0.041 μg·mL-1. In applications to quantitative analysis of nucleic acids synthesis samples, the recoveries of DNA or RNA ranged from 93.5% to 105.0%. Furthermore, the mechanism of interaction of pentamethyl-p-rosaniline hydrochloride 6B and nucleic acids system was also discussed.
林旭聪，谢增鸿*，郭良洽，刘绍锋，陈国南 . 核酸-甲基青莲6B分子作用体系的研究与应用 [J]. 光谱学与光谱分析, 2004, 24(02): 169-172.
LIN Xu-cong, XIE Zeng-hong, GUO Liang-qia, LIU Shao-feng, CHEN Guo-nan . Studies and Application of the Interaction of Pentamethyl-p-Rosaniline Hydrochloride 6B and Nucleic Acids System . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(02): 169-172.
[1] CHEN Guo-nan, WANG Jian. Analyst, 2000, 125：2294. [2] Hillisch A, Lorenz M, Diekmann S. Curr. Opin. Struct. Biol., 2001, 11(2): 201. [3] WU Ming-hu, SONG Gong-wu, LING Lian-sheng et al(吴鸣虎，宋功武，凌连生等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2000, 20(4): 575. [4] Ting Wang-ya，Zhao Lin-feng，Li Ke-an et al. Analytica Chimica Acta，1999, 396(1): 75. [5] XIE Zeng-hong, ZHANG Lan, HUANG Fu-xin(谢增鸿，张 兰，黄福新). Journal of Metallurgic Analysis(冶金分析)，1998, 18(3):30. [6] ZHENG Hong, WU Min, LI Dong-hui et al(郑 洪, 吴 敏, 李东辉等). Journal of Xiamen University(Natural Science) (厦门大学学报(自然科学版)), 2000, 39(2): 191. [7] Julia L Bricks, Juri L Slominskii, Margarita A Kudinova et al. Journal of Photochemistry and Photobiology A，Chemistry 2000, 132: 193. [8] XIANG Hai-yan, CHEN Xiao-ming, LI Song-qing et al(向海艳, 陈小明, 李松青等). Chinese Journal of Analytical Chemistry(分析化学), 2000, 28(11): 1398. [9] Yang Y, Jung D W, Bai D G et al. Electrophoresis, 2001, 22(5): 855. [10] WEI Yi-nan, LI Yuan-zong, CHANG Wen-bao(魏亦男, 李元宗, 常文保). Journal of Basic Science and Engineering(应用基础与工程科学学报), 1998, 6(4): 348.
