Abstract：The localization of the activity of immobilized urease on chitosan membrane was studied by X-ray microanalysis. BaCl2 and urea were selected as the capture and substrate respectively. The substrate was hydrolyzed by immobilized urease to produce NH3 and CO2 in Tris-HCl buffer(pH 7.0), and the latter was captured by BaCl2 to form precipitate. The precipite was deposited on the active site of immobilized urease. It is shown that the method is practicable and reliable. The optimum condition for the localization of activity of immobilized urease was studied.
马晓莉，姚子华*. 壳聚糖固定化脲酶活性的X射线微区分析[J]. 光谱学与光谱分析, 2005, 25(03): 456-459.
MA Xiao-li, YAO Zi-hua*. X-Ray Microanalysis of the Activity of Immobilized Urease on Chitosan Membrane . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 456-459.
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