Application of Synchronous and Three-Dimensional Fluorescent Spectrometry in Studies on Interaction Between Sunset Yellow and Human Serum Albumin
TANG Xiao-yu, LUO Yun-jing*, WEI An-qi, WANG Yan
Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
Abstract：Sunset Yellow (SY) is a potentially harmful artificial synthetic pigment. This paper, taking human serum albumin (HSA) as the research object, analyzes the quenching effect of SY on HSA and determines the quenching constant Ksv of the quenching reaction with the fluorescence spectroscopy method. The results show that the quenching effect of SY on HSA is static quenching. In addition, based on the analysis of the binding of SY and HSA with the synchronous fluorescence spectroscopy method, it is found that the binding of SY and HSA is near tryptophan, whose residue is caused to be in a stretching state and the structure of HSA has changed. Furthermore, this paper selects ten types of metal ion, adds them into the reaction system of SY and HSA under the simulated human physiological conditions, and detects the impacts of the metal ions on the system with the three-dimensional fluorescent spectrometry method, which demonstrating that Cu2+, Pb2+, Ni2+ and Mn2+ exert facilitation effects on the quenching process, and Ni2+ shows the largest impacts, with an increase of 22.6%, while Fe2+ and Zn2+ have inhibition effects on the quenching process, with an inhibition ratio of 14.12% and 14.2%, respectively. Therefore, the experimental method is applicable to the study on the toxicity of food additives and conducive to ensureing food safety as well as maintaining human health.
Key words：Sunset yellow; Human serum albumin; Fluorescent spectrometry；Quenching; Metal ion
汤小玉，罗云敬，魏安琪，王 妍. 同步与三维荧光光谱法研究日落黄和人血清蛋白的相互作用[J]. 光谱学与光谱分析, 2018, 38(10): 3129-3135.
TANG Xiao-yu, LUO Yun-jing, WEI An-qi, WANG Yan. Application of Synchronous and Three-Dimensional Fluorescent Spectrometry in Studies on Interaction Between Sunset Yellow and Human Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(10): 3129-3135.
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