CCD-Array Detection-Flow Injection Analysis of Oligomeric Proanthocyanidins in Health Food
LI Yuan-qian1, Lü Xing1, ZOU Xiao-li1, MI Jian-ping1, YANG Jing-guo2
1. Department of Sanitary Technology, West China School of Publish Health, Sichuan University, Chengdu 610041, China 2. College of Physical Science and Technology, Sichuan University, Chengdu 610041, China
Abstract：A flow-injection-CCD array detection spectrophotometry for the determination of oligomeric proanthocyanidin (OPC) in health foods was developed in the present paper, based on the reaction that OPC can form red anthocyanidin in hydrochloric acid medium with ferric ion as a catalytic agent. The absorption spectrum of OPC was scanned and the absorbance at 545 nm was determined by flow injection with CCD-array detector. The influencing factors of the chromogenic reaction and parameters of the flow injection analysis were optimized. There is a good linear relationship between the absorbances and the concentrations of OPC in the range of 0.010-0.20 mg·mL-1. The detection limit of the method was 5 μg·mL-1. The recoveries were 82.47%-98.18% with the relative standard deviation (RSD) of 1.5%-4.9%. The proposed method is sensitive, accurate and rapid with the sampling frequency of 10 samples·h-1 and suitable for the determination of OPC in the health food.
黎源倩1，吕 星1，邹晓莉1，米建萍1，杨经国2 . CCD阵列检测-流动注射分析保健食品中原花青素[J]. 光谱学与光谱分析, 2005, 25(10): 1724-1726.
LI Yuan-qian1, Lü Xing1, ZOU Xiao-li1, MI Jian-ping1, YANG Jing-guo2 . CCD-Array Detection-Flow Injection Analysis of Oligomeric Proanthocyanidins in Health Food. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(10): 1724-1726.
[1] YAO Kai, HE Qiang, Lü Yuan-ping, et al(姚 开，何 强，吕远平，等). Chemical Research and Application(化学研究与应用)，2002，14(2)：230. [2] ZHOU Ying, LI Yuan-qian, YANG Jing-guo, ZHENG Bo(周 颖, 黎源倩, 杨经国, 郑 波). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003, 23(2): 374. [3] LI Yuan-qian, YANG Jing-guo, ZHENG Xin, et al(黎源倩, 杨经国, 郑 欣, 等). Chinese Journal of Analytical Chemistry(分析化学)，1998, 26(7)：843. [4] YAO Kai, HE Qiang, Lü Yuan-ping, et al(姚 开，何 强，吕远平，等). Food and Ferment Industry(食品与发酵工业)，2002，28(3)：17. [5] FU Wu-sheng, CAI Yi-xin, LIN Li-yu, et al(傅武胜，蔡一新，林丽玉, 等). Food and Ferment Industry(食品与发酵工业)，2001，27(10)：57.
