Abstract：Direct fluorophotometric and flow-injection fluorophotometric methods for trace ammonia measurement involving the fluorescent reaction of ammonia with o-phthaldlaldehyde (OPA) and 2-mercaptoethanol(ME) producing derivant of iso-indol in alkaline solution are described. Triton X-100 was added to improve the stability of the system. The maximum excitation wavelength is 415 nm, while the maximum emission wavelength is 486 nm. With direct fliuorophotometric method, the calibration curve’s regression equation is ΔI＝22.286＋785.71cNH3(μｇ·mL-1)(r＝0.999 5) in the range of ammonia concentration from 0.2 to 1.0 μｇ·mL-1, and ΔI＝-27.429＋2 371.4cNH3(μｇ·mL-1) (r＝0.996 5) in the range of ammonia concentration from 0.02 to 0.10 μｇ·mL-1. With flow-injection fluorophotometric method, the calibration curve’s regression equation is ΔI＝1 188cNH3(μｇ·mL-1)(r＝0.999 8) in the range of ammonia concentration of ammonia 0-0.7 μｇ·mL-1. The mechanism of reaction was also briefly discussed.
郭良洽，谢增鸿*，林旭聪，柯子厚，陈国南 . 直接荧光法和流动注射荧光法测定微量氨的研究[J]. 光谱学与光谱分析, 2004, 24(07): 851-854.
GUO Liang-qia, XIE Zeng-hong*, LIN Xu-cong, KE Zi-hou, CHEN Guo-nan . Direct Fluorophotometric and Flow-Injection Fluorophotometric Methods for the Determination of Trace Ammonia . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(07): 851-854.
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