Study on Extracts of Active Substances from Larch Bark by FTIR Spectroscopy
CUI Xiao-xia1, ZHANG Xiao-li1, TANG Huan-wei1, ZHANG Li-ping1*, XU Chang-hua2, SUN Su-qin2
1. College of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China 2. Department of Chemistry, Tsinghua University, Beijing 100084, China
Abstract：The present study is to compare and analyze extracts of active substances from larch bark using ultrasonic wave quickly and undamagedly via Fourier transform infrared spectroscopy, second derivative IR spectroscopy and two dimensional spectroscopy. In the spectra of active substances from larch bark, there are four main components as the structural unit, and (+)-catechin, (-)-epicatechin, gallic acid and vitisinol represented a series of poly polyphenolic compounds. Furthermore the linkage between unit in proanthocyanidins dimmers was confirmed at position C(4)-C(8). Through observing the second derivative IR spectra of active substances from larch bark, the absorption peaks, 1 631, 1 561 and 1 469 cm-1 of active substances moved to the smaller wave number direction, while 1 606, 1 385, 1 285 and 1 157 cm-1 of active substances moved to the bigger wave number direction, and the intensity of characteristic peaks can represent the content of corresponding compound. In the two dimensional spectra, the active substances from larch bark have five automatic peaks in 1 345~1 675 cm-1. The authors developed the new method to analyse and evaluate the active substances from larch bark successfully.
崔晓霞1，张小丽1，唐焕威1，张力平1*，许长华2，孙素琴2 . 落叶松树皮活性物质提取及红外光谱分析[J]. 光谱学与光谱分析, 2012, 32(07): 1810-1814.
CUI Xiao-xia1, ZHANG Xiao-li1, TANG Huan-wei1, ZHANG Li-ping1*, XU Chang-hua2, SUN Su-qin2 . Study on Extracts of Active Substances from Larch Bark by FTIR Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(07): 1810-1814.
[1] SUN Da-wang, ZHAO Zu-chun, LUO Qing-yun(孙达旺，赵祖春，罗庆云). Chemistry and Industry of Forest Products(林产化学与工业), 1986, 6(1): 1. [2] SHEN Zhao-bang(沈兆邦). Chemistry and Industry of Forest Products(林产化学与工业), 1985, 5(1): 1. [3] Virgili F, Pagana G, Bourne L, et al. Free Radic. Biol. Med., 2000, 28(8): 1249. [4] Packer L, Rimbach G, Virgili F. Pycnogenol. Free Radic. Biol. Med., 1999, 27 (5/6): 704. [5] Stevenson, D E. Hurst, R D. Cell. Mol. Life Sci., 2007, 64: 2900. [6] Garcia-Marino M Rivas-Gonnalo J C, Ibanez E, et al. Anal. Chim. Acta, 2006, 563: 44. [7] Wu W, Sun S Q, Zhou Q, et al. Journal of Pharmaceutical and Biomedical Analysis, 2008, 46: 498. [8] XU Rong, SUN Su-qin, LIU You-gang(徐 荣，孙素琴，刘友刚). Chinese Journal of Analytical Chemistry(分析化学), 2009, 37(2): 221. [9] Zhou Q, Sun S Q, Yu L, et al. Journal of Molecular Structure, 2006, 799: 77. [10] Xu C H, Sun S Q, Guo C Q, et al. Vibrational Spectrosocopy, 2006, 41: 118. [11] Noda I. J. Am. Chem. Soc., 1989, 111: 8116. [12] Noda I. Appl. Spectrosc., 1990, 44: 550. [13] Noda I. Appl. Spectrosc., 1993, 47: 1329. [14] L Y Foo. Phytochemistry, 1981, 20(6): 1397. [15] Lai Yeap Foo，Lawrence J. Porter. Phytochemistry, 1980, 19(8): 1747.
