Abstract：Convolution spectrometry was applied to determining the DNA aberrance induced by UV-A, UV-B and UV-C radiation, and the extent of minute aberrance was quantitatively expressed by the differential value of convolution spectra. The differential values of DNA solutions irradiated by UV-A, UV-B and UV-C increase in turn, indicating an increscent DNA aberrance. The addition of dimethylsulfoxide or mannitol to DNA solution decreases the differential value in respective way under UV-A, UV-B and UV-C radiation, representing different inhibitory effects. Dimethylsulfoxide decreases the UV-A-induced DNA aberrance by 60%(approximate value), UV-C-induced aberrance by 35%(approximate value), and UV-B-induced aberrance by 20%(approximate value), while mannitol shows similar inhibitory effects (40%, approximate value) for UV-A, UV-B and UV-C -induced DNA aberrance. Although the extent of DNA aberrance varies, the distribution of differential points of convolution spectra reveals that the types of UV radiation-induced DNA aberrance are similar, and the inhibitory mechanism of two hydroxyl radical scavengers is possibly the same.
陆 峰，肖 杰，林培英，杨根金 . 褶合光谱法考察羟自由基清除剂对紫外照射致DNA变异的作用 [J]. 光谱学与光谱分析, 2004, 24(01): 82-84.
LU Feng, XIAO Jie, LIN Pei-ying, YANG Gen-jin. Convolution Spectrometric Determination of the Effect of Hydroxyl Radical Scavenger on UV Radiation-induced DNA Aberrance . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 82-84.
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