Near-Infrared Spectroscopy Technology for Online Monitoring of the Column Separation and Purification Process of Active Components of Centella asiatica L.Urban
LIU Hua1, YE Xiao-lan2, YANG Guang1, QI Yun-peng1*, FAN Guo-rong1*
1. Department of Pharmaceutical Analysis，School of Pharmacy，Second Military Medical University，Shanghai 200433，China 2. Department of Pharmaceutical Analysis，College of Pharmacy，Guangdong Pharmaceutical University，Guangzhou 510006，China
Abstract：The present paper is to study and develop a method for online monitoring of the column separation and purification process of active components that are madecassoside and asiaticoside of Centella asiatica L.Urban using near-infrared (NIR) spectroscopy technology. After collecting 50%-ethanol eluant, we detected their NIR spectra and developed the high performance liquid chromatography (HPLC) assay method of active components. Then, partial least square (PLS) was used to develop linear correlation between their NIR spectra and contents. During modeling, correlation coefficient (R2) and root mean square errors of cross-validation (RMSECV) were regarded as the indexes to select optimal wavenumbers and preprocessing methods. The optimal wavenumbers of madecassoside and asiaticoside were in the range of 12 000.8～7 499.8 cm-1 and 12 000.8～9 750.3 cm-1, respectively; R2 were 96.44 and 96.07, respectively, and RMSECV were 0.084 80 and 0.000 99, respectively. The above developed model was used for online monitoring of the contents of madecassoside and asiaticoside during the column separation and purification process of Centella asiatica L.Urban. The predicted results were satisfactory. This method was proved to be fast, convenient and precise. It can be used in online monitoring and quality control of the manufacturing of madecassoside and asiaticoside.
刘 桦1，叶晓岚2，杨 光1，亓云鹏1*，范国荣1* . 近红外光谱技术在线监测积雪草药材活性成分的大孔树脂分离纯化过程 [J]. 光谱学与光谱分析, 2013, 33(01): 98-101.
LIU Hua1, YE Xiao-lan2, YANG Guang1, QI Yun-peng1*, FAN Guo-rong1* . Near-Infrared Spectroscopy Technology for Online Monitoring of the Column Separation and Purification Process of Active Components of Centella asiatica L.Urban. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(01): 98-101.
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