Identification of Some Piper Crude Drugs Based on Fourier Transform Infrared Spectrometry
ZHOU Ye1, 2, ZHANG Qing-wei2, LUO Xue-jun3, LI Pei-fu2, SONG Heng2, ZHANG Bo-li1*
1. Tianjin University of Traditional Chinese Medicine，Tianjin 300193，China 2. College of Pharmacy，Tianjin Medical University，Key Laboratory of Tianjin Key Technologies of the Clinical Drug，Tianjin 300070，China 3. Tasly Research Institute of Chinese Medicine, Tianjin 300410, China
Abstract：The common peak ratio and variant peak ratio were calculated by FTIR spectroscopy of seven medicinal plants of Piper. The dual index sequence of common peak ratio and variant peak ratio was established, which showed the sibship of the medicinal plants. The common peak ratio of Piper kadsura (Choisy) Ohwi, Piper wallichii (Miq.) Hand.-Mazz. Piper laetispicum (C.DC.) was greater than 77%, and the variant peak ratio was less than 30%. The results showed the near sibship between the three drugs. The common peak ratio of Piper kadsura (Choisy) Ohwi, Piper nigrum L. and Piper boehmeriaefolium Wall (Miq.) C.DC. Var. tonkinense (C.DC.) was about 61% which showed the farther sibship. The common peak ratio of Piper kadsura (Choisy) Ohwi and Piper betle (Linn.) was only 44%, which showed the farthest sibship. Piper kadsura (choisy) Ohwi and its adulterants, such as Piper wallichii(Miq. ) Hand.-Mazz., Piper boehmeriaefolium Wall(Miq.) C.DC. Var. tonkinense C.DC., Piper laetispicum C.DC., Piper nigrum L., could be identified by comparing their second order derivative IR spectrum of the samples. FTIR technique is a non-destructive analysis method which provides information of functional group, type and hydrogen bond without complex pretreatment procedures such as extraction and separation. FTIR method has some characteristics such as rapid and simple analysis procedure, good reproducibility, non-destructive testing, few amount of required sample and low cost and is environment-friendly. The method solved the problems of limit in resource of Piper kadsura (Choisy) Ohwi, many fakes and difficulties in identification, and brought the security for the clinical medication. FTIR provides a new method for identification of Piper kadsura(choisy) Ohwi and its fakes and meets the requirement for comprehensive analysis and global analysis of traditional Chinese medicine.
Key words：Piper;Piper kadsura (choisy) Ohwi;IR fingerprint;Dual-index method;IR second derivative fingerprint
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