Comparative Study on the Infrared Fingerprint of Abrus Cantoniensis Based on the Methods of Sequential Analysis of Dual-Indexes and Cluster Analysis
KONG De-xin1, 2, HUANG Shu-shi2, HUANG Rong-shao1*, CHEN Xiu-li2, 3, WANG Yi-bing2, CHEN Zhi-cheng2, 3
1.College of Agronomy, Guangxi University, Nanning 530005, China 2.Lab of Biophysics, Guangxi Academy of Sciences, Nanning 530003, China 3.College of Physics and Electronic Engineering, Guangxi Normal University, Guilin 541004, China
Abstract：The methods of sequential analysis of dual-indexes and cluster analysis were utilized to investigate the infrared fingerprints of A.cantoniensis planted in different years and different places in Guangxi, China.The results showed that 6 samples were able to be completely separated only through 13 point smoothing, when the dual-indexes analysis was applied in the present research, and the accurate relationship between these samples could be inspected and expressed by quantitative relationships under 6-dimensional spaces; however, the effect of cluster was bad only through 13 point smoothing of raw spectra, and it was very difficult to find out the regular sequences while the cluster analysis was applied.Furthermore, the 6 samples were able to be completely separated if raw spectra were dealed with 1st derivative after 13 point smoothing, and the clustering effects were more obvious and 6 samples of A.cantoniensis were completely separated.The above two methods could be used to evaluate the quality of Chinese medicinal materials easily when the sample was not excessive quantitatively, but the method of dual-indexes analysis was more difficult than the clustering analysis if the sample size was too large, since a mass of data such as common peak ratio and variation peak ratio of the IR fingerprint spectra were processed and analyzed statistically, while this method could accurately find out the closest relationship between any samples through comparing the quantitative relationships of common peak ratio and variation peak ratio of each sample under 6-dimensional space; the precision of cluster analysis was less than dual-indexes analysis, but it was more convenient than dual-indexes analysis when large sample data were analysed.Finally the above two methods all showed that the chemical composition of the A.cantoniensis was similar in the same cultivated area, but the difference in chemical composition of A.cantoniensis in different years was distinct even they were in the same place.
孔德鑫1, 2，黄庶识2, 黄荣韶1*，陈秀丽2, 3，王一兵2，陈植成2, 3 . 基于双指标分析法和聚类分析法的鸡骨草红外指纹图谱比较研究[J]. 光谱学与光谱分析, 2010, 30(01): 45-49.
KONG De-xin1, 2, HUANG Shu-shi2, HUANG Rong-shao1*, CHEN Xiu-li2, 3, WANG Yi-bing2, CHEN Zhi-cheng2, 3 . Comparative Study on the Infrared Fingerprint of Abrus Cantoniensis Based on the Methods of Sequential Analysis of Dual-Indexes and Cluster Analysis . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(01): 45-49.
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