光谱学与光谱分析 |
|
|
|
|
|
Research on Molecular Spectra of Interactions Between Salvianolic Acid A and Salvianolic Acid B with Insulin, and Effect of Glucose on the Binding |
YANG Wen-yue1, CUI Lin1, QU Ling-ling1, XU Ning1, HUANG Yun1,2*, CUI Li-jian3*, ZHAN Wen-hong1, ZHAO Ding1 |
1. Pharmaceutical College, Hebei Medical University, Shijiazhuang 050017, China 2. Institute of Chinese Integrative Medicine, Hebei Medical University, Shijiazhuang 050017, China 3. Experimental Center, Hebei University of Chinese Medicine, Shijiazhuang 050091, China |
|
|
Abstract The interactions of Salvianolic acid A (SAA) and Salvianolic acid B (SAB) with insulin were studied by using fluorescence spectroscopy, UV-vis spectroscopy and ATR-FTIR spectroscopy in simulating physiological condition (pH 7.40). The fluorescence quenching of insulin by SAA and SAB were static quenching process. The results of synchronous fluorescence and three-dimensional fluorescence spectra suggested no obvious conformation changes of insulin after SAA or SAB binding. But ATR-FTIR spectra showed that SAA and SAB could change the secondary structures of insulin, of which β-turns decreased and random coil increased accompanied with α-helices and β-sheets no clear change. The glucose might influenced the the bioactivity of insulin in the SAA-insulin and SAB-insulin systems by changing the binding constants of SAA (or SAB) with insulin and exacerbating the changes of insulin conformation and relative contents of α-helices.
|
Received: 2015-05-06
Accepted: 2015-09-17
|
|
Corresponding Authors:
HUANG Yun, CUI Li-jian
E-mail: huangyun9317@126.com; cuilijianzy@126.com
|
|
[1] Zhang Mei, Li Xu, Qiu Genquan, et al. Jorunal of Chinese Medicinal Materials,2005, 28(6): 529. [2] Rong Xiuhua, Han Xuewen. Jorunal of Binzhou Medical College,1996, 19(6): 539. [3] Zhao Ling. Journal of Zhejiang College of Traditional Chinese Medicine,2009, 33(1): 82. [4] Wang Xiaomei, Zhen Zhuoli, Chen Xiaofen, et al. Journal of Hebei Medicine,2005, 11(9): 769. [5] Hye Sook Kang, Hae Young Chung, Dae Seok Byun, et al. Arehievs of Pharmacal Researeh,2003, 26(1): 24. [6] Fan Huaying, Fu Fenghua, Yang Mingyan, et al. Thrombosis Research,2010, 126(1): 17. [7] Huang Z S, Zeng C L, Zhu L J, et al. Journal of Thrombosis and Haemostasis,2010, 8(6): 1383. [8] Wang Shoubao, Tian Shuo, Fan Yang, et al. European Journal of Pharmacology,2009, 615(1-3): 125. [9] Guo Yongxue, Xiu Zhilong, Zhang Daijia, et al. Joumal of Pharmaceutical and Biomedical Analysis,2007, 43(4): 1249. [10] Yang Yanyi, Tian Ying, Tian Yanjiao, et al. Chinese Journal of Geriatric Care,2010, 8(4): 22. [11] Ye Yunhua. University Chemistry,2010, 25: 19. [12] Pullen R A, Lindsay D G, Stephen P Wood, et al. Nature, 1976, 259(5542): 369. [13] Kazarian S G, Chan K L A. Biochimica Et Biophysica Acta-Biomembranes,2006, 1758(7):858. [14] Hua Shi, Ling Xiong, Yang Kunyun, et al. Journal of Molecular Structure,1998, 446: 137. [15] Cui Fengling, Qin Lixia, Zhang Guisheng, et al. Journal of Pharmaceutical and Biomedical Analysis,2008, 48: 1029. [16] Li Qiang, Yang Wenyue, Qu Lingling, et al. Journal of Spectroscopy,2014, 834501:7. [17] Huang Yun, Cui Lijian, Wang Jianming, et al. Journal of Luminescence,2012, 132: 357. [18] Yang Jie, Qu Lingling, Yang Wenyue, et al. Journal of Spectroscopy,2014, 386586:9. [19] Huang Yun, Cui Lijian, Chen Chen, et al. Chinese Pharmacological Bulletin,2010, 26(6): 754. [20] Cui Fengling, Qin Lixia, Hu Xing, et al. Journal of Pharmaceutical and Biomedical Analysis,2008, 48: 1029. [21] Huang Yun, Cui Lijian, Wang Jianming, et al. European Journal of Medicinal Chemistry,2011, 46: 6039. [22] Zhang Xuan, Huang Lixin, Nie Songqing, et al. Journal of Chinese Pharmaceutical Sciences,2003, 12(1): 11. |
[1] |
ZHENG Hong-quan, DAI Jing-min*. Research Development of the Application of Photoacoustic Spectroscopy in Measurement of Trace Gas Concentration[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 1-14. |
[2] |
CHENG Jia-wei1, 2,LIU Xin-xing1, 2*,ZHANG Juan1, 2. Application of Infrared Spectroscopy in Exploration of Mineral Deposits: A Review[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 15-21. |
[3] |
FAN Ping-ping,LI Xue-ying,QIU Hui-min,HOU Guang-li,LIU Yan*. Spectral Analysis of Organic Carbon in Sediments of the Yellow Sea and Bohai Sea by Different Spectrometers[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 52-55. |
[4] |
LI Jie, ZHOU Qu*, JIA Lu-fen, CUI Xiao-sen. Comparative Study on Detection Methods of Furfural in Transformer Oil Based on IR and Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 125-133. |
[5] |
WANG Fang-yuan1, 2, HAN Sen1, 2, YE Song1, 2, YIN Shan1, 2, LI Shu1, 2, WANG Xin-qiang1, 2*. A DFT Method to Study the Structure and Raman Spectra of Lignin
Monomer and Dimer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 76-81. |
[6] |
BAI Xi-lin1, 2, PENG Yue1, 2, ZHANG Xue-dong1, 2, GE Jing1, 2*. Ultrafast Dynamics of CdSe/ZnS Quantum Dots and Quantum
Dot-Acceptor Molecular Complexes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 56-61. |
[7] |
XU Tian1, 2, LI Jing1, 2, LIU Zhen-hua1, 2*. Remote Sensing Inversion of Soil Manganese in Nanchuan District, Chongqing[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 69-75. |
[8] |
LIU Zhen1*, LIU Li2*, FAN Shuo2, ZHAO An-ran2, LIU Si-lu2. Training Sample Selection for Spectral Reconstruction Based on Improved K-Means Clustering[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 29-35. |
[9] |
YANG Chao-pu1, 2, FANG Wen-qing3*, WU Qing-feng3, LI Chun1, LI Xiao-long1. Study on Changes of Blue Light Hazard and Circadian Effect of AMOLED With Age Based on Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 36-43. |
[10] |
GAO Feng1, 2, XING Ya-ge3, 4, LUO Hua-ping1, 2, ZHANG Yuan-hua3, 4, GUO Ling3, 4*. Nondestructive Identification of Apricot Varieties Based on Visible/Near Infrared Spectroscopy and Chemometrics Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 44-51. |
[11] |
ZHENG Pei-chao, YIN Yi-tong, WANG Jin-mei*, ZHOU Chun-yan, ZHANG Li, ZENG Jin-rui, LÜ Qiang. Study on the Method of Detecting Phosphate Ions in Water Based on
Ultraviolet Absorption Spectrum Combined With SPA-ELM Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 82-87. |
[12] |
XU Qiu-yi1, 3, 4, ZHU Wen-yue3, 4, CHEN Jie2, 3, 4, LIU Qiang3, 4 *, ZHENG Jian-jie3, 4, YANG Tao2, 3, 4, YANG Teng-fei2, 3, 4. Calibration Method of Aerosol Absorption Coefficient Based on
Photoacoustic Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 88-94. |
[13] |
LI Xin-ting, ZHANG Feng, FENG Jie*. Convolutional Neural Network Combined With Improved Spectral
Processing Method for Potato Disease Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 215-224. |
[14] |
XING Hai-bo1, ZHENG Bo-wen1, LI Xin-yue1, HUANG Bo-tao2, XIANG Xiao2, HU Xiao-jun1*. Colorimetric and SERS Dual-Channel Sensing Detection of Pyrene in
Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 95-102. |
[15] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
|
|
|
|