| 光谱学与光谱分析 |
|
|
|
|
|
| Spectroscopic Characterization of Binding between Human Serum Albumin and 3,3’,4’,7-Tetradroxyflavone |
| SU Zhong1,2,XIE Meng-xia2*,LI Jian-dong1*,WANG Ying-dian2 |
1. Key Laboratory of Vegetation Ecology, MOE, Northeast Normal University, Changchun 130024, China
2. Analytical & Testing Center of Beijing Normal University, Beijing 100875, China |
|
|
|
|
Abstract The binding mechanism of human serum albumin with 3,3’, 4’,7-tetradroxyflavone (FIS) was characterized by fluorescence and UV absorption spectra. The intrinsic fluorescence of HSA was significantly quenched by FIS in the physiological condition (pH 7.4), and the quenching mechanism is mainly static quenching process in the drug to protein molar ratio ranging from 0.1 to 10. The results revealed that the drug and protein formed 1∶1 complex with the binding constants of (1.05±0.18)×105 L·mol-1. The dissociation behaviors of FIS in different pH conditions were investigated by UV absorption spectra, and it was found that FIS existed in the mixtures of ionic and neutral species. The UV absorption band Ⅰ of FIS has a significant red shift (above 40 nm) after combination with HSA, demonstrating that FIS was bound to protein in ionic species which was driven by electrostatic force. The second derivative spectra of FIS showed that the combination included specific and non-specific forms. The intrinsic fluorescence of FIS was conspicuously enhanced in the presence of HSA due to the excited-state proton transfer (ESPT) and this further confirmed the complex formation of HSA with FIS.
|
|
Received: 2006-10-19
Accepted: 2007-01-19
|
|
|
|
Corresponding Authors:
XIE Meng-xia,LI Jian-dong
E-mail: lijd@nenu.edu.cn
|
|
[1] Ma M, Hong C L, An S Q，et al. J. Agric. Food Chem.,2003, 51(8): 2390.
[2] Commenges D, Scotet V, Renaud S, et al. European Journal of Epideminol,2000, 16(4): 357.
[3] Jang H S, Kook S H, Son Y O. Biochim. Biophys. Acta，2005, 1726 (3): 309.
[4] Xie M X, Xu X Y, Wang Y D. Biochim. Biophys. Acta,2005, 1724(1): 215.
[5] LIU Luo-sheng, ZHANG Yu-yi, WANG Xing-po(刘洛生，张虞毅，王兴坡). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(9): 1490.
[6] WU Yun-xia, XING Da(吴云霞，邢 达). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(10): 1630.
[7] HE Hua, YE Hai-ying, DAI Li, et al(何 华，叶海英，戴 丽，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(3): 480.
[8] LIU Jia-qin, TIAN Jian-niao, BIAN Qing-quan, et al(刘家琴，田建袅，边清泉，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(4): 715.
[9] Liu Y, Xie M X, Kang J, et al. Spectrochim. Acta Part A,2003, 59(12): 2747.
[10] SHI Yan, YE Fan-di, ZHENG Wei-wan(石 燕，叶反帝，郑为完). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(7): 1314.
[11] Xie M X, Long M, Liu Y, et al. Biochim. Biophys. Acta,2006, 1760：1184.
[12] XIE Meng-xia, XU Xiao-yun, WANG Ying-dian, et al(谢孟峡，徐晓芸，王英典，等). Acta Chim. Sinica(化学学报)2005, 63(22): 2055.
[13] Kang J, Liu Y, Xie M X. Biochim. Biophys. Acta,2004, 1674：205.
[14] XIE Meng-xia, JIANG Min, LI Song, et al(谢孟峡，蒋 敏，李 崧，等). Acta Chim. Sinica(化学学报),2004, 62：1460.
[15] Guharay J, Dennison S M, Sengupta P K. Spectrochim. Acta Part A, 1999, 55: 1091. |
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [4] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
| [5] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [6] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [7] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [8] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
| [9] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [10] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
| [11] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [12] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [13] |
CHEN Wen-jing, XU Nuo, JIAO Zhao-hang, YOU Jia-hua, WANG He, QI Dong-li, FENG Yu*. Study on the Diagnosis of Breast Cancer by Fluorescence Spectrometry Based on Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2407-2412. |
| [14] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
| [15] |
LIU Xian-yu1, YANG Jiu-chang1, 2, TU Cai1, XU Ya-fen1, XU Chang3, CHEN Quan-li2*. Study on Spectral Characteristics of Scheelite From Xuebaoding, Pingwu County, Sichuan Province, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2550-2556. |
|
|
|
|
