| 光谱学与光谱分析 |
|
|
|
|
|
| Spectrometric Study on the Effect of Denaturant on the Conformation of Tartary Buckwheat Protein |
| GUO Xiao-na, YAO Hui-yuan |
| School of Foodstuff, Jiangnan University, Wuxi 214122, China |
|
|
|
|
Abstract The effect of ethanol and GuHCl solution on the structure of tartary buckwheat protein(TBWSP31)was studied by UV differential absorption and fluorescence emission spectra. The alcohol denaturation of TBWSP31 was a kind of partial denaturation. The hydrophobic core of TBWSP31 remained intact and the conformation of the hydrophilic shell was changed. When TBWSP31 was denatured by GuHCl solution with higher concentration, Tyr and Trp residues were exposed to the polar aqueous solvents from the hydrophobic core, and the microenvironment showed a great change.
|
|
Received: 2010-06-22
Accepted: 2010-09-15
|
|
|
|
Corresponding Authors:
GUO Xiao-na
E-mail: gxn1978@hotmail.com; xiaonaguo@jiangnan.edu.cn
|
|
[1] TAO Wei-sun, LI Wei, JIANG Yong-ming(陶慰孙, 李 惟, 姜涌明). Molecular Foundation of Protein(蛋白质分子基础). Beijing：Higher Education Press(北京: 高等教育出版社), 1995. 250.
[2] GUO Yao-jun(郭尧君). Fluorescence Experimental Techniques and Its Application in Molecular Biology(荧光实验技术及其在分子生物学中的应用). Beijing：Science Press(北京: 科学出版社), 1983. 121.
[3] Guo Xiaona, Zhang Hui, Yao Huiyuan. J. Agric. Food Chem., 2007, 55 (17): 6958.
[4] JIAO Jin-an, TANG Xiao-yi, ZHA Jing-juan(焦进安, 汤小仪, 查静娟). Acta Phytophysiologica Sinica(植物生理学报), 1986, 12(3): 266.
[5] GUO Wei, ZHENG Lü-yin, WU Yong-quan, et al(郭 维, 郑绿茵, 吴勇权, 等). Chinese Journal of Analytical Chemistry(分析化学), 2009, 3: 378.
[6] LI Ying-qi, YANG Bin-sheng(李英奇, 杨斌盛). Chinese Journal of Inorganic Chemsitry(无机化学学报)，2000，6：939.
[7] LIN Qing-song, YAN Qing, YAN Si-xu(林青松, 颜 青, 颜思旭). Journal of Xiamen University(厦门大学学报), 1995, 34(4): 608.
[8] LIN Qing-song, YAN Si-xu(林青松, 颜思旭). Journal of Xiamen University(厦门大学学报), 1994, 33(1): 100.
[9] HUA Yu-fei, GU Wen-ying, SHEN Pei-ying, et al(华欲飞, 谷文英, 沈培英, 等). Journal of Wuxi University of Light Industry(无锡轻工大学学报), 1996, 15(2): 129.
[10] Wetlaufer D B. Advance in Protein Chemistry, 1962，17：304.
[11] Glazer A N, Smith E L. J. Biol. Chem., 1961, 236: 2942.
[12] CHEN Qing-xi, YAN Si-xu(陈清西, 颜思旭). Chinese Biochemical Journal(生物化学杂志), 1991, 7(3): 301.
[13] WANG Shou-ye, XU Xiao-long, LIU Qing-liang, et al(王守业, 徐小龙, 刘清亮, 等). Progress in Chemistry(化学进展), 2001, 13(4): 257.
[14] Lakowicz J R. Principle of Fluorescence Spectroscopy. New York: Plenum Press, 1983. 341.
|
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
WANG Cai-ling1，ZHANG Jing1，WANG Hong-wei2*, SONG Xiao-nan1, JI Tong3. A Hyperspectral Image Classification Model Based on Band Clustering and Multi-Scale Structure Feature Fusion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 258-265. |
| [4] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [5] |
TIAN Fu-chao1, CHEN Lei2*, PEI Huan2, BAI Jie-qi1, ZENG Wen2. Study of Factors Influencing the Length of Argon Plasma Jets at
Atmospheric Pressure With Needle Ring Electrodes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3682-3689. |
| [6] |
WANG Ling-juan, OU Quan-hong, YAN Hao, TANG Jun-qi*. Preparation and Catalytic Properties of Gold Nanoflowers[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3747-3752. |
| [7] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
| [8] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [9] |
CHU Bing-quan1, 2, LI Cheng-feng1, DING Li3, GUO Zheng-yan1, WANG Shi-yu1, SUN Wei-jie1, JIN Wei-yi1, HE Yong2*. Nondestructive and Rapid Determination of Carbohydrate and Protein in T. obliquus Based on Hyperspectral Imaging Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3732-3741. |
| [10] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [11] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [12] |
ZHENG Ni-na1, 2*, XIE Pin-hua1, QIN Min1, DUAN Jun1. Research on the Influence of Lamp Structure of the Combined LED Broadband Light Source on Differential Optical Absorption Spectrum
Retrieval and Its Removing Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3339-3346. |
| [13] |
WAN Huang-xu1, 2, LIU Ji-qiang1*, HAN Xi-qiu1, 2, LIANG Jin-long2, ZHOU Ya-dong1, FAN Wei-jia1, WANG Ye-jian1, QIU Zhong-yan1, MENG Fan-wei3. Ultrastructure and Mineral Composition of Bathymodiolus Shell From Wocan-1 Hydrothermal Vent, Northwest Indian Ocean[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3497-3503. |
| [14] |
GUO Jing-fang, LIU Li-li*, CHENG Wei-wei, XU Bao-cheng, ZHANG Xiao-dan, YU Ying. Effect of Interaction Between Catechin and Glycosylated Porcine
Hemoglobin on Its Structural and Functional Properties[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3615-3621. |
| [15] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
|
|
|
|
