光谱学与光谱分析 |
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Spectrometric Study on the Effect of Denaturant on the Conformation of Tartary Buckwheat Protein |
GUO Xiao-na, YAO Hui-yuan |
School of Foodstuff, Jiangnan University, Wuxi 214122, China |
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Abstract The effect of ethanol and GuHCl solution on the structure of tartary buckwheat protein(TBWSP31)was studied by UV differential absorption and fluorescence emission spectra. The alcohol denaturation of TBWSP31 was a kind of partial denaturation. The hydrophobic core of TBWSP31 remained intact and the conformation of the hydrophilic shell was changed. When TBWSP31 was denatured by GuHCl solution with higher concentration, Tyr and Trp residues were exposed to the polar aqueous solvents from the hydrophobic core, and the microenvironment showed a great change.
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Received: 2010-06-22
Accepted: 2010-09-15
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Corresponding Authors:
GUO Xiao-na
E-mail: gxn1978@hotmail.com; xiaonaguo@jiangnan.edu.cn
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[1] TAO Wei-sun, LI Wei, JIANG Yong-ming(陶慰孙, 李 惟, 姜涌明). Molecular Foundation of Protein(蛋白质分子基础). Beijing:Higher Education Press(北京: 高等教育出版社), 1995. 250. [2] GUO Yao-jun(郭尧君). Fluorescence Experimental Techniques and Its Application in Molecular Biology(荧光实验技术及其在分子生物学中的应用). Beijing:Science Press(北京: 科学出版社), 1983. 121. [3] Guo Xiaona, Zhang Hui, Yao Huiyuan. J. Agric. Food Chem., 2007, 55 (17): 6958. [4] JIAO Jin-an, TANG Xiao-yi, ZHA Jing-juan(焦进安, 汤小仪, 查静娟). Acta Phytophysiologica Sinica(植物生理学报), 1986, 12(3): 266. [5] GUO Wei, ZHENG Lü-yin, WU Yong-quan, et al(郭 维, 郑绿茵, 吴勇权, 等). Chinese Journal of Analytical Chemistry(分析化学), 2009, 3: 378. [6] LI Ying-qi, YANG Bin-sheng(李英奇, 杨斌盛). Chinese Journal of Inorganic Chemsitry(无机化学学报),2000,6:939. [7] LIN Qing-song, YAN Qing, YAN Si-xu(林青松, 颜 青, 颜思旭). Journal of Xiamen University(厦门大学学报), 1995, 34(4): 608. [8] LIN Qing-song, YAN Si-xu(林青松, 颜思旭). Journal of Xiamen University(厦门大学学报), 1994, 33(1): 100. [9] HUA Yu-fei, GU Wen-ying, SHEN Pei-ying, et al(华欲飞, 谷文英, 沈培英, 等). Journal of Wuxi University of Light Industry(无锡轻工大学学报), 1996, 15(2): 129. [10] Wetlaufer D B. Advance in Protein Chemistry, 1962,17:304. [11] Glazer A N, Smith E L. J. Biol. Chem., 1961, 236: 2942. [12] CHEN Qing-xi, YAN Si-xu(陈清西, 颜思旭). Chinese Biochemical Journal(生物化学杂志), 1991, 7(3): 301. [13] WANG Shou-ye, XU Xiao-long, LIU Qing-liang, et al(王守业, 徐小龙, 刘清亮, 等). Progress in Chemistry(化学进展), 2001, 13(4): 257. [14] Lakowicz J R. Principle of Fluorescence Spectroscopy. New York: Plenum Press, 1983. 341.
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