| 光谱学与光谱分析 |
|
|
|
|
|
| Study on the Processing of Leech by FTIR and 2D-IR Correlation Spectroscopy |
| LI Bing-ning1, 2, WU Yan-wen2*, OUYANG Jie1, SUN Su-qin3, CHEN Shun-cong2 |
1. Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
2. Beijing Center for Physical and Chemical Analysis, Beijing 100089, China
3. Department of Chemistry, Tsinghua University, Beijing 100084, China |
|
|
|
|
Abstract The chemical differences of traditional Chinese medicine leech before and after processing were analyzed by FTIR and two-dimensional correlation infrared (2D-IR) spectroscopy. The result showed that the leech was high in protein, with characteristic peaks of amide Ⅰ, Ⅱ bands. Comparing the IR spectra of samples, the primary difference was that the characteristic peak of fresh leech was at 1 543 cm-1, while that of crude and processed leech was at 1 535 cm-1. A 2D-IR spectrum with heating perturbation was used to track the processing dynamics of leech. In the 2D-IR correlation spectra, fresh leech exhibited stronger automatic peaks of the amide Ⅰand Ⅱ bands than that of processed leech, which indicates that the protein components of the fresh leech were more sensitive to heat perturbation than the processed one. Moreover, the result of FTIR and 2D-IR correlation spectra validated that the 3-dimensional structure of protein was damaged and hydrogen bonds were broken after processing, which resulted in the inactivation of protein. The fatty acids and cholesterol components of leech were also oxidized in this process.
|
|
Received: 2010-07-12
Accepted: 2010-10-18
|
|
|
|
Corresponding Authors:
WU Yan-wen
E-mail: wuyanwenanal@hotmail.com
|
|
[1] Pharmacopiea Committee of the Ministry of Health of the People’s Republic of China(中华人民共和国国家药典委员会). Pharmacopiea the People’s Republic of China(中华人民共和国药典). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2010. 77.
[2] PAN He, GANG Hong-lin, WANG Zhi-guo(潘 贺, 刚宏林, 王志国). Acta Chinese Medicine and Pharmacology(中医药学报), 2006, 34(2): 60.
[3] LI Ming, WU Ji-biao, LIU Cong-cong(李 明, 武继彪, 刘聪聪). Traditional Chinese Medicinal Research(中医研究), 2006, 19(8): 62.
[4] Lü Wen-hai, WANG Qi(吕文海, 王 琦). Jorunal of Chinese Medicinal Materials(中药材), 1994, 19(4): 28.
[5] ZHANG Xi-chun(张锡纯). Discourses of Combining Chinese and Wastern Medicines(医学衷中参西录). Beijing: Peoples Medical Publishing House(北京: 人民卫生出版社), 1987. 383.
[6] Monreal M, Costal J, Salva P. Drugs Aging, 1996, 8(3): 171.
[7] Liu Hongxia, Sun Suqin, Lü Guanghua. Vib. Spectrosc., 2006, 40: 202.
[8] Wu Yanwen, Sun Suqin, Zhao Jing. Journal of Molecular. Structure, 2008, 882(1-3): 107.
[9] TANG Hui-ying, YAN Dan, WU Yan-wen(唐慧英，鄢 丹，武彦文). World Science Technology-Modernization of Traditional Chinese Medicine(世界科学技术-中医药现代化), 2009, 2: 283.
[10] OU Chang-xing, CHEN Ke-yi (欧长兴, 陈可冀). The Clinical Progress on the Leech(水蛭的临床进展). Beijing: Ancient Chinese Medical Book Press(北京: 中医古籍出版社), 1994.
[11] YAN Long-fei, SUN Zhi-rong(阎隆飞, 孙之荣). Moleculer Structure of Protein(蛋白质分子结构). Beijing: Tsinghua Univesity Press(北京: 清华大学出版社), 1999. 16.
[12] LIU Li-fang, JIN Rong-luan, XU Guo-jun, et al(刘丽芳, 金荣鸾, 徐国钧, 等). Chinese Traditional Patent Medicine(中成药), 2001, 23(12): 880.
[13] SUN Su-qin, ZHOU Qun, QIN Zhu(孙素琴, 周 群, 秦 竹). Atlas of Two-Dimensional Correlation Infrared Spectroscopy for Traditional Chinese Medicine Identification(中药二维相关红外光谱鉴定图集). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2003. 154.
[14] WENG Shi-fu(翁诗甫). The Fourier Transform Infrared Spectroscopy(傅里叶变换红外光谱仪). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2005. 253.
[15] XIE Jing-xi, CHANG Jun-biao, WANG Xu-ming(谢晶曦, 常俊标, 王绪明). The Infrared Spectroscopy in Organic Chemistry and Drug Chemistry(红外光谱在有机化学和药物化学中的应用). Beijing: Science Press(北京: 科学出版社), 2001. 304.
|
| [1] |
LI Jie, ZHOU Qu*, JIA Lu-fen, CUI Xiao-sen. Comparative Study on Detection Methods of Furfural in Transformer Oil Based on IR and Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 125-133. |
| [2] |
CHENG Jia-wei1, 2，LIU Xin-xing1, 2*，ZHANG Juan1, 2. Application of Infrared Spectroscopy in Exploration of Mineral Deposits: A Review[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 15-21. |
| [3] |
YANG Cheng-en1, 2, LI Meng3, LU Qiu-yu2, WANG Jin-ling4, LI Yu-ting2*, SU Ling1*. Fast Prediction of Flavone and Polysaccharide Contents in
Aronia Melanocarpa by FTIR and ELM[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 62-68. |
| [4] |
GAO Feng1, 2, XING Ya-ge3, 4, LUO Hua-ping1, 2, ZHANG Yuan-hua3, 4, GUO Ling3, 4*. Nondestructive Identification of Apricot Varieties Based on Visible/Near Infrared Spectroscopy and Chemometrics Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 44-51. |
| [5] |
LIU Jia, ZHENG Ya-long, WANG Cheng-bo, YIN Zuo-wei*, PAN Shao-kui. Spectra Characterization of Diaspore-Sapphire From Hotan, Xinjiang[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 176-180. |
| [6] |
BAO Hao1, 2，ZHANG Yan1, 2*. Research on Spectral Feature Band Selection Model Based on Improved Harris Hawk Optimization Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 148-157. |
| [7] |
GUO Ya-fei1, CAO Qiang1, YE Lei-lei1, ZHANG Cheng-yuan1, KOU Ren-bo1, WANG Jun-mei1, GUO Mei1, 2*. Double Index Sequence Analysis of FTIR and Anti-Inflammatory Spectrum Effect Relationship of Rheum Tanguticum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 188-196. |
| [8] |
CHU Bing-quan1, 2, LI Cheng-feng1, DING Li3, GUO Zheng-yan1, WANG Shi-yu1, SUN Wei-jie1, JIN Wei-yi1, HE Yong2*. Nondestructive and Rapid Determination of Carbohydrate and Protein in T. obliquus Based on Hyperspectral Imaging Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3732-3741. |
| [9] |
LI Xiao-dian1, TANG Nian1, ZHANG Man-jun1, SUN Dong-wei1, HE Shu-kai2, WANG Xian-zhong2, 3, ZENG Xiao-zhe2*, WANG Xing-hui2, LIU Xi-ya2. Infrared Spectral Characteristics and Mixing Ratio Detection Method of a New Environmentally Friendly Insulating Gas C5-PFK[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3794-3801. |
| [10] |
HU Cai-ping1, HE Cheng-yu2, KONG Li-wei3, ZHU You-you3*, WU Bin4, ZHOU Hao-xiang3, SUN Jun2. Identification of Tea Based on Near-Infrared Spectra and Fuzzy Linear Discriminant QR Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3802-3805. |
| [11] |
LIU Xin-peng1, SUN Xiang-hong2, QIN Yu-hua1*, ZHANG Min1, GONG Hui-li3. Research on t-SNE Similarity Measurement Method Based on Wasserstein Divergence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3806-3812. |
| [12] |
SUN Wei-ji1, LIU Lang1, 2*, HOU Dong-zhuang3, QIU Hua-fu1, 2, TU Bing-bing4, XIN Jie1. Experimental Study on Physicochemical Properties and Hydration Activity of Modified Magnesium Slag[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3877-3884. |
| [13] |
BAI Xue-bing1, 2, SONG Chang-ze1, ZHANG Qian-wei1, DAI Bin-xiu1, JIN Guo-jie1, 2, LIU Wen-zheng1, TAO Yong-sheng1, 2*. Rapid and Nndestructive Dagnosis Mthod for Posphate Dficiency in “Cabernet Sauvignon” Gape Laves by Vis/NIR Sectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3719-3725. |
| [14] |
WANG Qi-biao1, HE Yu-kai1, LUO Yu-shi1, WANG Shu-jun1, XIE Bo2, DENG Chao2*, LIU Yong3, TUO Xian-guo3. Study on Analysis Method of Distiller's Grains Acidity Based on
Convolutional Neural Network and Near Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3726-3731. |
| [15] |
DANG Rui, GAO Zi-ang, ZHANG Tong, WANG Jia-xing. Lighting Damage Model of Silk Cultural Relics in Museum Collections Based on Infrared Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3930-3936. |
|
|
|
|
