光谱学与光谱分析 |
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The Molecular Composition and Spectral Properties of Polysaccharide Isolated from Pu-Erh Tea and Its Material |
GONG Jia-shun1, HU Xiao-jing1, PENG Chun-xiu2, ZHOU Hong-jie3 |
1. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China 2. College of Horticulture and Landscape,Yunnan Agricultural University, Kunming 650201, China 3. College of Pu-erh Tea, Yunnan Agricultural University, Kunming 650201,China |
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Abstract Pu-erh tea, a kind of well-known tea from the ancient time, is originally produced in the Yunnan Lanchan River basin through a special solid state fermentation by fungi. It uses sun-dried green tea as its starting materials. To investigate the variation of composition and spectral properties of polysaccharide during solid state fermentation of pu-erh tea by using Saccharomyces cerevisiae as preponderant starter and using sun-dried green tea as materials in the present study. The results showed that the content of water soluble polysaccharide was increased,and the activity of hydrolase such as cellulase,pectinase and glucomylase were also enhanced. The content of neutral sugar increased with the ferment time increasing and the MW of raw polysaccharide showed significant difference during fermentation. The main polysaccharide TPS2 and TPS1 were isolated and purified from pu-erh tea and its materials by DEAE-52 and Sephadex G-150 column chromatography. TPS2 contains the higher content of uronic acid, but TPS1 contains the higher contents of neutral sugar and protein. Monosaccharide analysis by GC-MS revealed that TPS1 and TPS2 were composed of arabinose, galactose, glucose, rhamnose, xylose and mannose with molar ratios of 24.2∶23.6∶5.9∶3.2∶1.8∶1.1 and 19.3∶26.9∶3.2∶2.7∶1.3∶5.5, respectively. The average molecular weight of TPS1 and TPS2 was 1.68×104 and 1.21×104 Daltons, respectively. UV scanning spectrum showed that TPS1 and TPS2 had no characteristic absorption between 200 and 400 nm wavelength, it suggested that they contain trace protein. IR spectrum of TPS1 and TPS2 demonstrated that pyranoid rings were contained in them. As shown in the image of atomic force microscope, the molecular appearance of TPS1 and TPS2 resembled islands and apparently consisted of conglomerations. The height of conglomerations of TPS2 was about 40nm and the length or width was 0.5-0.8 μm, while the height of conglomerations of TPS1 was about 4nm and the length or width was 0.2-0.4 μm. TPS2 shows sheet conglomerations with rough surface, but TPS1 shows squama conglomerations with smooth surface in the image of scanning electron micrograph. The experimental data suggested that the variation of composition and spectral properties of polysaccharide isolated from pu-erh tea and its materials owed to the action of microorganism and humid and thermal action for long time process.
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Received: 2009-10-09
Accepted: 2010-01-12
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Corresponding Authors:
GONG Jia-shun
E-mail: gong199@163.com
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[1] ZHOU Hong-jie, GONG Jia-shun, WANG Xing-yin,et al(周红杰,龚加顺,王星银,等). Yunnan Pu-erh Tea(云南普洱茶). Kunming: Yunnan Science and Technoloy Press(昆明:云南科技出版社),2004. 50. [2] Duh P D, Yen G C, Yen W J, et al. Journal of Agricultural and Food Chemistry, 2004,52:8169. [3] Wu She-Ching, Yen Gow-Chin, Wang Bor-Sen, et al. LWT -Food Science and Technology,2007,40:506. [4] Thales R, Caroline G, Lauro Ml. American Chemical Society and American Society of Pharmacognosy,2006, 69(7): 1018. [5] CHEN Xiao-qiang,YE Yang,CHENG Hao,et al(陈小强,叶 阳,成 浩,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2009,29(4):1083. [6] GONG Jia-shun, ZHOU Hong-jie, ZHANG Xin-fu,et al(龚加顺,周红杰,张新富,等). Journal of Tea Science(茶叶科学),2005,25(4):300. [7] Dubois M, Gilles K A, Hamilton J K, et al. Analytical Chemistry,1956,28:350. [8] LI Jian-wu(李建武). Experimental Principle and Method of Biochemistry(生物化学实验原理和方法). Beijing: Peking University Press(北京:北京大学出版社), 2001. 181. [9] Dische Z. Journal of Biological Chem., 1997,272: 189. [10] Rong L, Takashi Y. Carbohydrate Polymers, 2003, 54: 419. [11] ZHOU Peng, SHEN Jin-can, XIE Ming-yong(周 鹏,沈金灿,谢明勇). Journal of Xiamen University(Natural Science)(厦门大学学报·自然科学版),2003,42(2):213. [12] Kumar S, Chaudhury K, Sen P, et al. J. Nanobiotechnology, 2005, 27: 3. [13] QU Ping, CHEN Guan-hua, WU Guo-jiang, et al(屈 平,陈冠华,吴国江,等). Journal of Chinese Electron Microscopy Society(电子显微学报),2006, 25:175. [14] NI De-jiang, CHEN Yu-qiong, XIE Bi-jun, et al(倪德江,陈玉琼,谢笔钧,等). Chemical Journal of Chinese Universities(高等学校化学学报),2004,25(12):2263.
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