光谱学与光谱分析 |
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Spectral Analysis of Cyanobacteria Chlorophyll in Polluted Wate |
ZHAO Zhi-min1,2, HONG Xiao-qin1, LI Peng1,JIN Xiao-dong1 |
1. College of Science, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China 2. CSIRO Materials Science and Engineering, 37 Graham Road, Highett, VIC 3190, Australia |
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Abstract he polluted water with abundant nourishment cause phytoplankton, such as cyanobacteria, to grow rapidly, which brings great harm to environment. In the present paper, the absorption spectrum of cyanobacteria was measured and analyzed in order to estimate the content of the chlorophyll accurately. The same amount of cyanobacteria was separately cultured in pure water and lake water for different time. The chlorophyll was extracted from the cyanobacteria for the same time by 95% of ethanol. Then the ethanol extract was tested by ultraviolet visible spectrometry. The results show that the absorption spectrum of the chlorophyll has three absorption peaks at 279.5,436.0 and 664.5 nm respectively. However, the absorbency at 279.5 nm cannot reflect the content of the chlorophyll. The absorbencies at 436.0 and 664.5 nm have linear relationship with the content of chlorophyll. Moreover, the dispersion between the absorbency at 436.0 nm and the absorbency at 664.5 nm can reflect the content of chlorophyll more accurately. The research provides the experimental and theoretical basis for the highly accurate detection of the water quality.
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Received: 2009-08-10
Accepted: 2009-11-12
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Corresponding Authors:
ZHAO Zhi-min
E-mail: zhaozhimin@nuaa.edu.cn
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[1] HE Jun-hua, CHENG Yong-jin, ZHANG Hao(贺俊华,程永进,张 昊). Optics & Optoelectronic Technology(光学与光电子技术), 2007, 5(5): 16. [2] MA Rong-hua, DAI Jin-fang(马荣华,戴锦芳). Journal of Remote Sensing(遥感学报), 2005, 9(1): 78. [3] Iluz D, Yacobi Y Z, Gitelson A. Int. J. Remote Sensing, 2003, 24(5): 1157. [4] Michael Schagerl, Gabriela Künzl. Biologia, 2007, 62(3): 270. [5] LI Zhen-guo, LU Jun, WANG Guo-xiang, et al(李振国,卢 军,王国祥,等). Environmental Monitoring in China(中国环境监测), 2006, 22(2): 21. [6] TANG Yao-ji, YOU Wen-wei, CHEN Ying, et al(唐尧基,游文玮,陈 莹,等). Analytical Instrument(分析仪器), 2004, (3): 24. [7] ZHAO Zhi-min, XIN Yu-jun, WANG Le-xin, et al(赵志敏, 辛玉军, 王乐新, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(1): 138. [8] ZHU Wei-hua, ZHAO Zhi-min, GUO Xin, et al(朱卫华,赵志敏, 郭 昕). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2009, 29(4): 1004. [9] WANG Le-xin, ZHAO Zhi-min, XIN Yu-jun, et al(王乐新,赵志敏, 辛玉军,等). Applied Laser(应用激光), 2007, 27(4): 333. [10] ZHU Wei-hua,ZHAO Zhi-min,GUO Xin,et al(朱卫华,赵志敏,郭 昕,等). Journal of Life Sciences, 2008, 2(1): 62. [11] Millership J S, Parker C, Donnelly D. Il Farmaco, 2005, 60(4): 333. [12] Bebawy Lories I, Abbas Samah S, Fattah Laila A, et al. Il Farmaco, 2005, 60(10): 859. [13] Wortmann A C, Froehlich P E, Pinto R B, et al. Spectroscopy, 2007, 21(3): 161. [14] Lababpour Abdolmajid, Lee Choul-Gyun. Journal of Bioscience and Bioengineering, 2006, 101(2): 104. [15] Stauffer Mark T, Hunter Leland J, Troncone Steven K. Spectroscopy Letters, 2007, 40(3): 429. [16] Wu Nan, Zhu Yongqang, Browns, et al. Lab. Chip, 1009, 9(23): 3391. [17] Chawla H M, Mrig Sarika. Journal of Analytical Chemistry, 2009, 64(6): 585. [18] CHEN Ting, HUANG Zhi-yong, DAI Yu-lan, et al(陈 婷, 黄志勇, 戴玉兰, 等). Journal of Chinese Insitute of Food Science and Technology(中国食品学报), 2008, 8(6): 149. [19] Thongboonkerd Visith, Songtawee Napat, Kanlaya Rattiyaporn, et al. Analytical and Bioanalytical Chemistry, 2006, 384(4): 964. [20] WANG Tianhu, ZHAO Zhimin, ZHANG Lin, et al. Journal of Molecular Structure, 2009, 937(1-3): 65. [21] CHEN Guo-zhen(陈国珍). Ultraviolet Visible Spectrophotometry, Volume 1(紫外-可见光分光光度法, 上册). Beijing: Atomic Energy Press(北京:原子能出版社), 1983. 42. [22] LU Dong-yu, CUI Xin-tu, HUANG Jing-rong, et al(卢东昱,崔新图,黄镜荣,等). College Physics(大学物理),2006, 25(1): 50.
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