| 光谱学与光谱分析 |
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| Spectroscopic Studies on the Interactions of a Hexadeca-Carboxy Zinc Phthalocyanine and Serum Albumin and Preparation of the Bioconjugates |
| CHEN Yan-mei1, HUANG Jian-dong1*, LIU Feng-ran1, SUN Rui-qing1, Dennis K P Ng2 |
1. College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China
2. Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, China |
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Abstract The interactions of the new photosensitizer {2,3,9,10,16,17,23,24-octakis[(3,5-dicarboxy)phenoxy]-phthalocyaninato}zinc(Ⅱ) [ZnPc(COOH)16] and serum albumins (BSA and HSA) were investigated by absorption and fluorescence spectroscopy. The binding constants were found to be 2.25-2.94×106 L·mol-1. The 1∶1 ZnPc(COOH)16-BSA and ZnPc(COOH)16-BSA conjugates were also prepared by incubating a mixture of ZnPc(COOH)16 and the corresponding serum albumin in 2∶1 mole ratio followed by separation by gel permeation chromatography. The Q band absorption and fluorescence emission of ZnPc(COOH)16 are slightly red-shifted in the conjugates, and the spectral features suggest that the phthalocyanine exists predominantly in monomeric form in the conjugates.
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Received: 2005-04-12
Accepted: 2005-07-26
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Corresponding Authors:
HUANG Jian-dong
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| Cite this article: |
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CHEN Yan-mei,HUANG Jian-dong,LIU Feng-ran, et al. Spectroscopic Studies on the Interactions of a Hexadeca-Carboxy Zinc Phthalocyanine and Serum Albumin and Preparation of the Bioconjugates [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(08): 1387-1391.
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https://www.gpxygpfx.com/EN/Y2006/V26/I08/1387 |
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