| 光谱学与光谱分析 |
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| The Secondary Structure of Winter-Wheat-Bran Antifreeze Protein Determined by FTIR and CD Spectrum |
| ZHANG Chao1, 2, ZHANG Hui2*, ZHAO Xiao-yan1, MA Yue1, YAO Hui-yuan2 |
1. National Vegetable Engineering Research Center, Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
2. Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China |
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Abstract The secondary structure of winter wheat bran antifreeze protein was analyzed by FTIR and CD spectra. The characteristic peaks of the secondary structure of the winter wheat bran antifreeze protein were overlapped from the FTIR analysis. The overlapped peaks of amide Ⅰ and Ⅲ of the winter wheat bran antifreeze protein were fitted by the Gauss peak to show the characteristic peaks of the α-helix, β-sheet, β-turn and random coil. The CD spectrum was evaluated by the Chen-Yang principle and analyzed by the least squares method. Furthermore, the difference between the two analyses was discussed. The final results showed that the α-helix, β-sheet, β-turn and random coil of the winter wheat bran antifreeze protein was 15.05%-15.87%, 36.00%-36.92%, 17.31%-17.75%, and 30.30%-30.82%, respectively. The result would be helpful to the study of the antifreeze mechanism of the winter wheat bran antifreeze protein.
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Received: 2008-06-16
Accepted: 2008-09-18
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Corresponding Authors:
ZHANG Hui
E-mail: hui1003@hotmail.cm
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