| 光谱学与光谱分析 |
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| Determination of Nucleic Acids Using Toluidine Blue as a Fluorescence Probe |
| GUO Liang-qia,YE Fang-gui,LIN Xu-cong,XIE Zeng-hong* |
| Department of Chemistry, Fuzhou University, Fuzhou 350002, China |
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Abstract A new fluorimetric method has been developed for the determination of deoxyribonucleic acid (DNA) with toluidine blue(TB) as a fluorescence probe. It is based on the fluorescence quenching of toluidine blue in the presence of DNA. In Tris-HCl buffer solution (pH 8.5), the calibration graph was linear over the range 0.10-6.00 μg·mL-1 for ctDNA, and the detection limit was 27 ng·mL-1. The result of the determination of DNA of camphor tree’s leaf by this method was satisfactory.
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Received: 2004-06-26
Accepted: 2004-11-15
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Corresponding Authors:
XIE Zeng-hong
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| Cite this article: |
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GUO Liang-qia,YE Fang-gui,LIN Xu-cong, et al. Determination of Nucleic Acids Using Toluidine Blue as a Fluorescence Probe[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 121-124.
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https://www.gpxygpfx.com/EN/Y2006/V26/I01/121 |
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