| 光谱学与光谱分析 |
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| Fluorescence Reaction of the System of Safranine T-Sodium Dodecylbenzene Sulfonate-Protein and Its Application |
| CHENG Ding-xi, WANG Shao-hua |
| Key Laboratory of Environmental Science and Engineering，Education Commission of Henan, Department of Chemistry, Henan Normal University, Xinxiang 453007, China |
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Abstract There is a self-polymerization equilibrium system between the cation of the fluorescent dye-safranine T and its dimer. In the presence of appropriate amounts of anionic surfactant SDBS, the above mentioned equilibrium can be adjusted by a quantitative addition of protein, thus leading to a regular change in the fluorescence intensity of the system. Based on this phenomenon, the properties of the absorption spectrum and fluorescence spectrum of the system were studied, and a new fluorescence probe for the determination of protein was established. It was found that ST was apt to dimerize in the solution added with appropriate amounts of anionic surfactant. Because of the dimerization, its fluorescence intensity decreased, whereas the addition of protein caused the dimer to be depolymerized and the system fluorescence increased. A linear relationship between the fluorescence intensities and serum albumin concentration was found in the range of 0-40 μg·mL-1,and the detection limit found was 0.034 μg·mL-1. The significant features of this method were its rapidity of reaction, and high sensitivity, accuracy and selectivity. The method was applied to the determination of total proteins in real human serum, and satisfactory results were obtained.
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Received: 2005-09-16
Accepted: 2005-12-26
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Corresponding Authors:
CHENG Ding-xi
E-mail: cdxhsd@163.com
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| Cite this article: |
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CHENG Ding-xi,WANG Shao-hua. Fluorescence Reaction of the System of Safranine T-Sodium Dodecylbenzene Sulfonate-Protein and Its Application[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(01): 104-107.
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https://www.gpxygpfx.com/EN/Y2007/V27/I01/104 |
[1] GUAN Zheng-da(管正大). Handbook of Clinical Purpose of Medical Inspection(医学检验临床意义手册). Beijing：The People’s Press of Surgeon(北京：人民军医出版社)，1994. 176.
[2] ZHANG Ning, TANG Tie, HAO Dong-mei，et al(张 宁，唐 铁，郝冬梅，等). Chinese J. of Chromatogram(色谱)，1998，16(3)：195.
[3] ZHAO Chang-rong, LIU Bao-sheng, ZHANG Hong-yi(赵长容，刘保生，张红医). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(1)：92.
[4] MA Chun-qi, LI Ke-an, TONG Shen-yang. Anal. Chim. Acta, 1996, 333(1-2): 83.
[5] FENG Ning-chuan, HE Su-ping, ZHANG Juan, et al(冯宁川，何苏萍，张 娟，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(2)：194.
[6] Shealy D B, Lipowaka M, Lipowski J. Anal. Chem., 1995, 67: 247.
[7] GAO Feng, ZHU Chang-qing, WANG Lun(高 峰，朱昌青，王 伦). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(1)：85.
[8] GAO Feng, WANG Lun(高 峰，王 伦). Physical Testing and Chemical Analysis Part B: Chemical Analysis(理化检验-化学分册)，2004，40(7)：395.
[9] ZHAO Guo-xi(赵国玺). Physical Chemistry of Surfactant，2nd ed.(表面活性剂物理化学·第2版). Beijing: Peking University Press(北京：北京大学出版社)，1991. 165.
[10] Bradford M M. Anal. Biochem., 1976, 72: 248. |
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