| 光谱学与光谱分析 |
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| Spectra of in situ Reduction of Ag(I) by Phycocyanin and Dynamic Formation of Ag(0) |
| YANG Fang1, 2,GUO Zhen-jiang1,BAI Yan1,ZHENG Wen-jie1, 2* |
1. Department of Chemistry, Jinan University, Guangzhou 510632, China
2. Institute of Hydrobiology, Jinan University, Guangzhou 510632, China |
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Abstract The interaction of Ag(I) and Phycocyanin(PC for short) was investigated by ultraviolet-visible, fluorescence and infrared spectra under the condition of 4 ℃ and without light. The results showed that the characteristic absorption of PC at 615 nm decreased gradually with the increase in Ag(I) concentration and interaction time. Accordingly, its fluorescence emission peak and two fluorescence excitation peaks also decreased. Dynamic formation of nano-Ag(0) was also observed by synchronous fluorescence spectra, showing that Ag(0) was formed by the PC in situ reduction. It was observed by TEM that Ag(0) dispersed in PC, which formed a bioconjugate with PC as the core and nano-Ag(0) as the crust. The bioconjugate was round and its diameter was about 15-30 nm.
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Received: 2005-11-16
Accepted: 2006-03-06
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Corresponding Authors:
ZHENG Wen-jie
E-mail: tzhwj@jnu.edu.cn
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| Cite this article: |
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YANG Fang,GUO Zhen-jiang,BAI Yan, et al. Spectra of in situ Reduction of Ag(I) by Phycocyanin and Dynamic Formation of Ag(0)[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(01): 23-27.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2007/V27/I01/23 |
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