| 光谱学与光谱分析 |
|
|
|
|
|
| Determination of Thirty Three Elements in Lung Cancer Tissues of Patients with Lung Cancer by Microwave Digestion-ICP-MS |
| ZHANG Lin-lin1, MA Qian-li2, HUANG Yun-chao2, WU Guo-ping3, WEI Fu-sheng3* |
1. The Civil and Environment Engineering School of University of Science & Technology Beijing，Beijing 100083, China
2. The Third Affiliated Hospital of Kunming Medical College, Kunming 650031, China
3. China National Environmental Monitoring Center, Beijing 100012, China |
|
|
|
|
Abstract A method for determining 33 elements in lung tissues of patients with lung cancer was developed by using vacuum freeze-drying microwave digestion-ICP-MS. The lung tissue samples were treated by vacuum freeze-drying equipment. After microwave digestion in HNO3-H2O2 solution system, the samples were diluted with the method of constant volume. Under the optimized conditions the samples were analyzed by ICP-MS. The double internal standard elements Rh and Re were used to compensate for matrix suppression effect and sensitivity drift. The analytical results showed that the detection limits of the 33 elements were 0.01-0.45 ng·mL-1. The national standard reference material GBW(E)080193 bovine liver was analyzed by the described method and the measured element values accorded with the standard values or the reference values. The relative standard deviation (RSD) of the method was 2.1%-14.3%. The recovery rates of the studied elements were 90.1%-117.5%. The contents of 33 elements in lung cancer tissues, paracancerous lung tissues and benign lung tissues of 6 patients with lung cancer were determined by the method. It was indicated that the method is rapid, simple and accurate for determining multi-elements in human lung tissue and other biological samples.
|
|
Received: 2008-12-10
Accepted: 2009-03-20
|
|
|
|
Corresponding Authors:
WEI Fu-sheng
E-mail: weifsh@cae.cn
|
|
[1] HE Xing-zhou(何兴舟). Chinses Journal of Epidemiology(中华流行病学杂志), 2003, 24(10): 857.
[2] YAN Shi-ming, WU Jing-bing, XU De-yang, et al(颜世铭, 吴敬炳, 徐德扬, 等). Trace Elements Introduction(微量元素导论). Shanghai: Tongji University Press((上海: 同济大学出版社), 1992. 257.
[3] Reddy S Bhuloka, Charles M John, Raju G J Naga, et al. Nuclear Instruments and Methods in Physics Research, 2003, 207: 345.
[4] Patriarca Marita. Microchemical Journal, 1996, 54: 262.
[5] CHEN Hang-ting, CAO Shu-qin, ZENG Xian-jin, et al(陈杭亭，曹淑琴，曾宪津，等). Chinese Journal of Analytical Chemistry(分析化学)，2001，29(5)：592.
[6] Javis K E, Gray A L, Houk R S. Handbook of Inductively Coupled Plasma Mass Spectrometry(ICP-MS手册). Translated by YIN Ming, LI Bing(尹 明，李 冰, 译). Beijing：Atomic Energy Press(北京: 原子能出版社)，1997. 113.
|
| [1] |
XU Tian1, 2, LI Jing1, 2, LIU Zhen-hua1, 2*. Remote Sensing Inversion of Soil Manganese in Nanchuan District, Chongqing[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 69-75. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [4] |
HE Yan1, TAO Ran1, YANG Ming-xing1, 2*. The Spectral and Technology Studies of Faience Beads Unearthed in Hubei Province During Warring States Period[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3700-3709. |
| [5] |
LIU Hong-wei1, FU Liang2*, CHEN Lin3. Analysis of Heavy Metal Elements in Palm Oil Using MP-AES Based on Extraction Induced by Emulsion Breaking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3111-3116. |
| [6] |
WANG Wei-en. Analysis of Trace Elements in Ophiocordyceps Sinensis From
Different Habitats[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3247-3251. |
| [7] |
JIN Xu-guang1, 2, WANG Jin-zhuan1, 2*, LI Bei1, 2, QUE Wan-ting1, 2, WANG Liang1, 2, ZHANG Fan1, 2, ZHANG Chi1, ZHOU Jun-gui1, FU Rong-jin1. Quantification of Au in Gold Ornaments Obtained by Different Electroformed Process[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2755-2760. |
| [8] |
WANG Yan1, HUANG Yi1, 2*, YANG Fan1, 2*, WU Zhong-wei2, 3, GUAN Yao4, XUE Fei1. The Origin and Geochemical Characteristics of the Hydrothermal Sediments From the 49.2°E—50.5°E Hydrothermal Fields of the Southwest Indian Ocean Ultra-Slow Spreading Ridge[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2868-2875. |
| [9] |
CHEN Chao-yang1, 2, LIU Cui-hong1, 2, LI Zhi-bin3, Andy Hsitien Shen1, 2*. Alexandrite Effect Origin of Gem Grade Diaspore[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2557-2562. |
| [10] |
ZHANG Hao-yu1, FU Biao1*, WANG Jiao1, MA Xiao-ling2, LUO Guang-qian1, YAO Hong1. Determination of Trace Rare Earth Elements in Coal Ash by Inductively Coupled Plasma Tandem Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2074-2081. |
| [11] |
CHEN Di, SONG Chen, SONG Shan-shan, ZHANG Zhi-jie*, ZHANG Hai-yan. The Dating of 9 Batches of Authentic Os Draconis and the Correlation
Between the Age Range and the Ingredients[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1900-1904. |
| [12] |
TANG Quan1, ZHONG Min-jia2, YIN Peng-kun2, ZHANG Zhi3, CHEN Zhen-ming1, WU Gui-rong3*, LIN Qing-yu4*. Imaging of Elements in Plant Under Heavy Metal Stress Based on Laser-Induced Breakdown Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1485-1488. |
| [13] |
LAI Si-han1, LIU Yan-song1, 2, 3*, LI Cheng-lin1, WANG Di1, HE Xing-hui1, LIU Qi1, SHEN Qian4. Study on Hyperspectral Inversion of Rare-Dispersed Element Cadmium Content in Lead-Zinc Ores[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1275-1281. |
| [14] |
WU Lei1, LI Ling-yun2, PENG Yong-zhen1*. Rapid Determination of Trace Elements in Water by Total Reflection
X-Ray Fluorescence Spectrometry Using Direct Sampling[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 990-996. |
| [15] |
ZHANG Zhi-wei1, 2, QIU Rong1, 2*, YAO Yin-xu1, 2, WAN Qing3, PAN Gao-wei1, SHI Jin-fang1. Measurement and Analysis of Uranium Using Laser-Induced
Breakdown Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 57-61. |
|
|
|
|
