光谱学与光谱分析 |
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Study of Scavenging Activity of Sorghum Pigment to Hydroxyl Free Radicals by Fluorimetry |
ZHANG Hai-rong,WANG Wen-yan |
Lab of Biochemical Analysis, Xinzhou Teacher’s University, Xinzhou 034000, China |
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Abstract A natural product, sorghum pigment, consists of a number of important flavonoid derivatives, occurrs on the seed capsules or in the stems of many sorghums, and is widely applied in different fields of food, cosmetic and dyeing industries, It is important for scavenging hydroxyl free radicals and protection of human healthiness. Scavenging capacities of hydroxyl free radicals with sodium nitrite, quercetin and sorghum pigment were comparatively researched by fluorimetry, and the model of hydroxyl free radicals produced is based on the reaction of Cu2+-catalyzed oxidation of ascorbic acid in the presence of hydrogen peroxide. The hydroxyl radicals react with benzoic acid, forming a fluorescent product, and the fluorescence intensity was determined by the concentration of hydroxybenzoic acid. The experimental results show that the sodium nitrite, quercetin and sorghum pigment have a quantity-effect relationship for scavenging hydroxyl free radicals, and sodium nitrite and quercetin in comparison with sorghum pigment have high antioxidant capacity. Finally, the quenching mechanisms were explored with sodium nitrite, sorghum pigment, and quercetin respectively. The sorghum pigment and sodium nitrite feature a dynamic quenching processes, while quercetin shows a static quenching processes. A reference method was provided for reasonable exploitation and utilization of sorghum pigment.
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Received: 2006-01-08
Accepted: 2006-04-16
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Corresponding Authors:
ZHANG Hai-rong
E-mail: biochem@xztc.edu.cn
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Cite this article: |
ZHANG Hai-rong,WANG Wen-yan. Study of Scavenging Activity of Sorghum Pigment to Hydroxyl Free Radicals by Fluorimetry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(03): 547-551.
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URL: |
https://www.gpxygpfx.com/EN/Y2007/V27/I03/547 |
[1] ZHANG Zhao-jun, XIAO Li-juan(张兆俊,肖丽娟). Food and Grease(粮食与油脂), 2005, (5): 40. [2] WANG Qing-bin, CHEN Guo-liang(王清滨, 陈国良). Food Colorant and Its Analytical Methods, First Edition(食品着色剂及其分析方法·第1版). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2004. 53. [3] JIA Zhi-shen, WU Jian-min, TANG Meng-cheng(贾之慎, 邬建敏, 唐孟成). Progress of Biochemistry and Biophysics(生物化学与生物物理进展), 1996, 23(2): 184. [4] ZHANG Li-wei, WANG Jin-shan, JIANG Chong-qiu(张立伟, 王金山, 江崇球). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(9): 1103. [5] CHEN Guan-hua, TIAN Yi-ling, YANG Geng-liang, et al(陈冠华, 田益玲, 杨更亮, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002,22(4): 634. [6] TIAN Yi-ling, CHEN Guan-hua, CUI Tong(田益玲, 陈冠华, 崔 同). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(4): 617. [7] Gutteridge J M C. Biochemical J., 1987, 243: 709. [8] QU Peng, LI Guan-lian, XU Mao-tian(瞿 鹏, 李贯良, 徐茂田). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(11): 1407. [9] YANG Pin(杨 频). Introduction of Bioinorganic Chemistry(生物无机化学导论). Xi’an: Press of Xi’an Jiaotong University, First edition(西安: 西安交通大学出版社·第1版), 1989. 154. [10] ZHANG Hai-rong,GUO Si-yuan, LI Lin,et al(张海容, 郭祀远, 李 琳, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(6): 829.
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