| 光谱学与光谱分析 |
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| The Establishment of the Method of the Fiber Optic Chemical Sensor Synchronous Absorption-Fluorescence |
| ZHANG Li-hua1, Arkin IBURAIM2* |
1. Department of Pharmacy of Maternal and Child Health Care of Zaozhuang, Zaozhuang 277100, China
2. Central Laboratory of Xinjiang Medical University, Urumqi 830011, China |
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Abstract A new method of simultaneously measuring fiber-optic chemical sensor absorption spectrum and fluorescence spectrum is established. Make synchronous absorption-fluorescence cuvette, establish synchronous absorption-fluorescence spectrometry instrumentation combined by fiber optic chemical sensor technology, measure the synchronous absorption-fluorescence spectrums of solutions of rhodamine B, vitamin B2 and vitamin B6, compared by absorption spectroscopy measured by traditional UV-Visible photometric method and fluorescence spectroscopy measured by traditional fluorescence method. Synchronous absorption-fluorescence method measure absorption spectrums and fluorescence spectrums the same to traditional photometric and fluorescence spectroscopy of rhodamine B, vitamin B2 and vitamin B6. The maximum wavelength of fluorescence intensity method has high accuracy relatively compared with fluorescence, but the maximum wavelength of absorption has a slight deviation. Synchronous absorption-fluorescence method means simultaneously measure the absorption spectrums and fluorescence spectrums of the fluorescent substance, making two spectrums to one. The method measured the maximum emission wavelength with high accuracy, though in measuring maximum absorption wavelength there is a slight deviation, but it is worth further studying.
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Received: 2014-12-26
Accepted: 2015-04-15
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Corresponding Authors:
Arkin IBURAIM
E-mail: arkinxyd@sina.com
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