| 光谱学与光谱分析 |
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| Fluorescence Spectra Study of a New Toxic Protein from Malania Oleifera |
| YUAN Yan1, 2,XIAO Han1,KANG Hong-jun1,CHEN Rui-yan1,DAI Xiao-chang1* |
1. Key Laboratory of Medical Chemistry for Natural Resource, Ministry of Education, School of Chemical Science and Technology, Yunan University, Kunming 650091, China
2. Department of Life Science and Technology, Kunming University, Kunming 650031, China |
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Abstract A new toxic protein named malanin was isolated from seeds of Malania oleifera by hydrophobic chromatography, whose cytotoxic activities against carcinoma cells were very strong. The conformational changes of malanin at various temperatures, pH, organic solvents, surfactant, denaturant and fluorescence quenching solvents were studied by fluorescence spectra. The fluorescence spectra of malanin excited at 280 nm and 295 nm showed a maximum at 340 nm. The emission spectra of malanin showed that Trp residues were located by a great degree in the hydrophobic area. Addition of SDS, CH5N3·HSCN, acrylamide and KI led to changes in the molecular conformation of malanin, and caused the fluorescence quenching of Trp residues. The red-shifted emission band of malanin after adding CH5N3·HSCN showed that Trp residues were exposed in polar solvents.
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Received: 2008-02-22
Accepted: 2008-05-26
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Corresponding Authors:
DAI Xiao-chang
E-mail: xchdai@ynu.edu.cn
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[1] QIU Hua-xing, LIN You-run(丘华兴, 林有润). Flora of China(中国植物志). Beijing: Science Press(北京: 科学出版社), 1988. 24, 34.
[2] FU Li-guo(傅立国). China Plant Red Book Rare and Endangered Plant Species(中国植物红皮书-稀有濒危植物). Beijing: Science Press(北京: 科学出版社), 1992. 1, 480.
[3] WANG Cai-ming, HUANG Shi-xun, WANG Yan(王才明, 黄仕训, 王 燕). Guihaia(广西植物), 1994, 14(3): 277.
[4] WANG Shou-ye, YU Hua-ming(王守业, 余华明). Acta Biochimica et Biophysica Sinica(生物化学与生物物理学报), 1998, 30(3): 303.
[5] XU Jin-gou, WANG Zun-ben(许金钩, 王尊本). Fluorescence Analytical Approach(荧光分析法, 第3版). Beijing: Science Press(北京: 科学出版社), 2006.
[6] WEI Xiao-fang, DING Xi-ming, LIU Hui-zhou (魏晓芳, 丁西明, 刘会洲). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2000, 20(4): 556.
[7] FENG Shang-yuan, CHEN Rong, YANG Wen-qin, et al(冯尚源, 陈 荣, 杨文琴, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(11): 2279.
[8] YAN Yuan, LIU Tao, HUANG Jian-feng(鄢 远, 刘 韬, 黄坚锋). Acta Chimica Sinica(化学学报), 1997, 55: 1214.
[9] CHEN Zhen-long, ZHAO Da-qing, LIU Qing-liang(陈真龙, 赵大庆, 刘清亮). Chinese Journal of Inorganic Chemistry(无机化学学报), 1999, 15(2): 185.
[10] ZHANG Jing(张 静). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(4): 455.
[11] Lü Hong-zhou, LIU Chao, BAO Jin-ku(吕鸿周, 刘 超, 鲍锦库). Journal of Sichuan University(Natural Science Edition)(四川大学学报·自然科学版), 2006, 43(6): 1389.
[12] WANG Ke-yi, SUN Jian-zhong(王克夷, 孙建忠). Acta Biochimica et Biophysica Sinica(生物化学与生物物理学报), 1994, 26(6): 649.
[13] YAN Cheng-nong, XIE Ze-yu, MEI Ping, et al(颜承农, 谢泽钰, 梅 平, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(12): 2542.
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