同步与三维荧光光谱法研究日落黄和人血清蛋白的相互作用

doi:10.3964/j.issn.1000-0593(2018)10-3129-07

Abstract
References
Related Citation (15)

Download: PDF (5127 KB)
Export: BibTeX | EndNote (RIS)

Abstract Sunset Yellow (SY) is a potentially harmful artificial synthetic pigment. This paper, taking human serum albumin (HSA) as the research object, analyzes the quenching effect of SY on HSA and determines the quenching constant Ksv of the quenching reaction with the fluorescence spectroscopy method. The results show that the quenching effect of SY on HSA is static quenching. In addition, based on the analysis of the binding of SY and HSA with the synchronous fluorescence spectroscopy method, it is found that the binding of SY and HSA is near tryptophan, whose residue is caused to be in a stretching state and the structure of HSA has changed. Furthermore, this paper selects ten types of metal ion, adds them into the reaction system of SY and HSA under the simulated human physiological conditions, and detects the impacts of the metal ions on the system with the three-dimensional fluorescent spectrometry method, which demonstrating that Cu²⁺, Pb²⁺, Ni²⁺ and Mn²⁺exert facilitation effects on the quenching process, and Ni²⁺shows the largest impacts, with an increase of 22.6%, while Fe²⁺ and Zn²⁺have inhibition effects on the quenching process, with an inhibition ratio of 14.12% and 14.2%, respectively. Therefore, the experimental method is applicable to the study on the toxicity of food additives and conducive to ensureing food safety as well as maintaining human health.

Key words：Sunset yellow; Human serum albumin; Fluorescent spectrometry；Quenching; Metal ion

Received: 2017-10-23 Accepted: 2018-02-18

ZTFLH:

TS201.6

Corresponding Authors: LUO Yun-jing E-mail: luoyj@bjut.edu.cn

Cite this article:

URL:

https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2018)10-3129-07 OR https://www.gpxygpfx.com/EN/Y2018/V38/I10/3129

[1] National Standards of the People’s Republic of China(中华人民共和国国家标准). GB 6227.1—2010. Food Additives Technology Sunset Yellow(食品添加剂日落黄). Beijing: Standards Press of China(北京：中国标准出版社)，2010.
[2] Mccann D, Barrett A, Cooper A, et al. Lancet, 2007, 370(9598): 1560.
[3] LI Wei-xue, ZHU Guo-hua, Lü Dong-jun, et al(李伟雪，朱国华，吕东军，等). Chemical Research and Application(化学研究与应用). 2017, 29(6): 806.
[4] Ajmal M R, Zaidi N, Alam P, et al. Journal of Biomolecular Structure & Dynamics, 2017, 35(1): 46.
[5] He L L, Wang Z X, Wang Y X, et al. Colloids & Surfaces B Biointerfaces, 2016, 145: 820.
[6] Atanu Singha Roy, Amit Kumar Dinda, Nitin Kumar Pandey. Journal of Pharmaceutical Analysis, 2016, 256.
[7] Chen G, Zhang Q, Cheng Y, et al. Spectrochimica Acta- Part A: Molecular and Biomolecular Spectroscopy, 2013, 115(4): 269.
[8] JIANG Yi-yu, WANG Xiang-li, WANG Hua-hua(江怿雨, 王湘利, 汪华华，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2016, 36(9)：2894.
[9] LI Jin-huang, CHEN Yi-ting, ZHENG Jing-jing(李金凰，陈毅挺，郑晶晶). Analysis and Testing Technology and Instruments(分析测试技术与仪器)，2017, 23(1): 6.
[10] ZHANG Fang-yuan, NI Yong-nian(张方圆, 倪永年). Acta Chimica Sinica(化学学报), 2012, 70(12): 1379.
[11] Manjushree M, Revanasiddappa Hosakere D. Journal of Molecular Liquids，2017，237: 23.
[12] DENG Shao-dong, SHUAI Ou, LIN Li, et al(邓少东, 帅欧, 林励, 等). Chinese Pharmacological Bulletin(中国药理学通报)，2012, 28(11): 1620.
[13] GUO Ming, HUANG Feng-qin, LI Ming-hui, et al(郭明, 黄凤琴, 李铭慧, 等). Journal of Agricultural Biotechnology(农业生物技术学报), 2014, 22(7): 892.
[14] CHEN Yao, SUN Peng, LIU Mai-li, et al(陈瑶, 孙鹏, 刘买利, 等). Chinese Journal of Magnetic Resonance(波谱学杂志)，2017, 34(3): 266.