光谱学与光谱分析 |
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Study on the Influence of Temperature on Near Infrared Spectra |
JIANG Huan-yu,XIE Li-juan,PENG Yong-shi,YING Yi-bin* |
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China |
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Abstract Near-infrared (NIR) spectroscopy technique is one of qualitative and quantitative analysis techniques that were developed quickly in recent years. However, the characteristic of NIR spectral region determines its low absorbance, low sign-to-noise and peak overlapping, which results in the phenomenon that th NIR spectrum is affected by sample source, sample varieties, sample state, location and sample temperature and uncertainty of spectrum. In this paper, the influence of temperature on NIR spectra was studied and calibration and validation models of chlorophyll at different temperatures were built. The results show that temperature does have effect on the model precision. High precision could be achieved at 10 and 20 ℃ and the former was the best though using more principal components. Calibration and validation precisions of models were poor when it was 25 ℃. At the same time, spectra obtained at different temperatures were classified using discriminate analysis. It was found that the spectra were misclassified exception ones obtained at 20 ℃ which meant that there were not clear differences between the spectra exception the ones at 20 ℃. The primary research work about the condition and application of detecting leaves was studied which helps to provide theory foundation for proposing temperature modification model in the future.
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Received: 2007-09-08
Accepted: 2007-12-18
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Corresponding Authors:
YING Yi-bin
E-mail: ybying@zju.edu.cn
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