光谱学与光谱分析 |
|
|
|
|
|
Study on Conformation Transitions of Trichokonin Ⅵ, a Peptaibol-Like Antimicrobial Peptide, in Different Solvents by Circular Dichroism Spectroscopy |
DONG Xiao-wei1, 4, SONG Xiao-yan2, 3*, SHI Mei2, ZHANG Yu-zhong2 |
1. School of Life Sciences, Shandong Normal University, Ji’nan 250114, China 2. State Key Laboratory of Microbial Technology, Shandong University, Ji’nan 250100, China 3. School of Medical and Life Science, Jinan University, Ji’nan 250223, China 4. Technology Center, Shandong Tobacco Industry Corporation, Ji’nan 250013, China |
|
|
Abstract Trichokonin Ⅵ, a peptaibol-like antimicrobial peptides isolated from the cultured substrates of trichoderma koningii SMF2, has 20 amino acid residues. The conformational flexibility of trichokonin Ⅵ in organic solvents with different polarities, aqueous solvents and membrane mimic solvents was studied by circular dichroism spectroscopy. Trichokonin Ⅵ takes on a typical α-helical structure in different organic solvents, but helicity decreases in aqueous solvent. The helical content increases with increasing the concentration of TFE up to 30%. In phosphate buffered saline, the CD spectrum of trichokonin Ⅵ is concentration dependent, and the intensity of the peaks increases with increasing the concentration of trichokonin Ⅵ. SDS induces a significant transition towards a helix formation, and the CD spectra in membrane mimic solvents increase helicity compared with those recorded without membrane mimic solvents, suggesting the interaction of the peptides with the membrane.
|
Received: 2009-09-09
Accepted: 2009-12-08
|
|
Corresponding Authors:
SONG Xiao-yan
E-mail: chm_songxy@ujn.edu.cn
|
|
[1] SONG Xiao-yan, SHEN Qing-tao, XIE Shu-tao, et al(宋晓妍,沈庆涛,解树涛,等). Chemistry of Life Science(生命的化学),2005,25(3): 184. [2] Chugh J K, Wallace B A. Biochemical Society Transaction, 2001, 29: 565. [3] Whitmore L, Wallace B A. The European Physical Journal, 2004, 33: 233. [4] Chen L L, Liu L J, Shi M, et al. FEMS Microbiology Letters, 2009,299(2): 135. [5] Song X Y, Shen Q T, Xie S T, et al. FEMS Microbiology Letters, 2006, 260: 119. [6] Song X Y, Xie S T, Chen X L, et al. Journal of Biotechnology, 2007, 131(2): 209. [7] Bodkin M J, Goodfellow J M H. Biopolymers, 1996, 39: 43. [8] Luo P, Baldwin R L. Biochemistry, 1997, 36: 8413. [9] Blondelle S E, Lohner K, Aguilar M. Biochimica et Biophysica Acta, 1999, 1462: 89. [10] Mao D, Wachter E, Wallace B A. Biochemistry, 1982, 21: 4960. [11] Chugh J K, Bruckner H, Wallace B A. Biochemistry, 2002, 41: 12934. [12] Duclohier H, Alder G M, Bashford C L, et al. Biophysical Journal, 2004, 87: 1705. [13] Duclohier H, Snook C F, Wallace B A. Biochimica et Biophysica Acta, 1998, 1415: 255. [14] Grigoriev P A, Kronen M, Schlegel B, et al. Bioelectrochemistry, 2002, 57: 119. [15] O’Reilly A O, Wallace B A. Journal of Peptide Science, 2003, 9: 769. [16] Sansom M S, Balaram P, Karle I L. The European Physical Journal, 1993, 21: 369. [17] Reusser F. Journal of Biology Chemistry, 1967, 242: 243. [18] Sharman G J, Try A C, Williams D H, et al. Biochemical Journal, 1996, 320 (Pt3): 723.
|
[1] |
LI Chen-xi1, SUN Ze-yu1, 2, ZHAO Yu2*, YIN Li-hui2, CHEN Wen-liang1, 3, LIU Rong1, 3, XU Ke-xin1, 3. The Research Progress of Two-Dimensional Correlation Spectroscopy and Its Application in Protein Substances Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 1993-2001. |
[2] |
ZHU Xiang1, 2, YUAN Chao-sheng2, LIANG Yong-fu2, WANG Zheng2, LI Hai-ning2, HUANGFU Zhan-biao1, ZHOU Song1, ZHOU Bo1, DONG Xing-bang1, CHENG Xue-rui2*, YANG Kun1*. Study on the Effect of Cooling Rate on Crystallization Process and
Product of [C12mim][BF4] Melt Based on POM, Raman and SAXS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 801-805. |
[3] |
ZHANG Meng-jie, CAO Si-min, WANG Meng-yu, LI Hao-yang, LI Dong, ZHAO Ze-nan, XU Jian-hua*. Fluorescence Enhancement and Conformational Studies of Coenzyme NADH With Aluminum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(04): 997-1003. |
[4] |
ZENG Jian-hua, MENG Yan, LIU Lin-lin, YANG Yang, LI Mei-ying, WANG Zi-yue, ZHU Xiu-qing*, SHI Yan-guo*. Characterization and Effects of pH on the Conformation of Hemp Protein Isolate Based on Multi-Spectroscopic Technique[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(12): 3748-3754. |
[5] |
WANG Xiao-xia1*, NIE Zhi-hua2, MA Li-tong1, CUI Jin-long1, SAI Hua-zheng1,ZHAO Wen-yuan1. Study on the Interaction Between Minocycline and Bovine Serum Albumin by Multi Spectral Method and Molecular Docking Simulation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1503-1508. |
[6] |
GAO Nan1, TU Qian2, SUI Cheng-hua2, 3. Study on Different Conformation Raman Spectra of Methamphetamine Based on Density Functional Theory[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(03): 744-749. |
[7] |
REN Yu-fen, CHENG Xue-rui*, WANG Yong-qiang, YANG Kun, YUAN Chao-sheng, ZHANG Meng-wei. Research on Hydrogen Bonds and Conformations of n-Pentanol under High Pressure[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(03): 694-699. |
[8] |
HUANG Chao-bo3, XU Han3, YANG Ming-guan3, LI Zhen-jing1, 3, YANG Hua1, 3, WANG Chang-lu1, 2, 3, ZHOU Qing-li1, 2, 3*. The Interaction between Rubropunctamine and Bovine Serum Albumin Using Spectrometry and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 3102-3108. |
[9] |
TANG Qian1, 3*, GONG Ting-ting1,3, CAO Hong-yu1, 3, WANG Li-hao2,3, LI Ru-yu2, ZHENG Xue-fang1, 2, 3*. Mechanism of the Variation of Horseradish Peroxidase Catalytic Activity Induced by Light[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(12): 3692-3698. |
[10] |
SUN Ning-jie1, CAI Xiao-li1, ZHANG Yue-fei1, CHI Ru-an1*, Yunjie Xu2*. The Conformation of Sophocarpidin by Vibrational Circular Dichroism and DFT Calculations[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(09): 2806-2813. |
[11] |
XU Kun1, 2, 3, WANG Ju-lin1, 2, 3*, HE Qiu-ju4. The Influence of Alum in Alum Gelatin Solution on Cellulose,Calcium Carbonate and Gelatin in XUAN Paper[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1829-1833. |
[12] |
WANG Hao1, 2, HUANG Yan2, LIU Zhi-tong2, GAO Zhen-hua2, SUN Da-rui2, TAO Ye2*. Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(07): 2110-2114. |
[13] |
CAI Jian-zhou1, ZHANG Wu2* . Studies on the Interaction of Poly-L-lysine with Fibrinogen with Spectroscopic Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(01): 166-171. |
[14] |
HU Yong, WU Xiao-yong*, ZHONG Nan-jing, XU Jin-rui . Study on the Conformation of Soybean Selenoprotein Solution with Spectroscopy Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(09): 2874-2878. |
[15] |
HU Tao-ying1, WANG Yi-run1, ZHOU Shan-shan1,2, LIU Ying1,2* . Investigation of the Interaction between Perfluorododecanoic Acid and Human Serum Albumin by Multi-Spectroscopic and Molecular Modeling Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(07): 2330-2336. |
|
|
|
|