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| Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer |
| WANG Hao1, 2, HUANG Yan2, LIU Zhi-tong2, GAO Zhen-hua2, SUN Da-rui2, TAO Ye2* |
1. Materials Science and Energy, Institute of Physics and Material Science, Anhui University, Hefei 230601, China
2. Beijing Synchrotron Radiation Facility (BSRF), Institute of High Energy Physics (IHEP), Chinese Academy of Sciences, Beijing 100049, China
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Abstract The setup of dynamic synchrotron radiation dichroism spectroscopy (SRCD)is discussed by using continuous flow probe with microfluidic mixer, developed at 4B8 ultraviolet vacuum spectroscopy beamline at Beijing Synchrotron Radiation Facility. The quartz microfluidic mixer was fabricated with deep ion etching with channel depth down to 44.5 microns. The mixer is designed on serpentine configuration to reach the effective mixing. The mixing efficiency was evaluated based on fluorescence image of the mixing solution under practical high viscous solution condition. Dynamic SRCD measurement with mixer was validated after implementing focus position feedback, since wavelength dispersion of the focusing lens results in the changes of focus spot position during wavelength scan. The time range covers from 4.5 to 270 ms at present under 500 (L·min-1 flow rate within the wide observation channel. The performance of the time-resolved SRCD is demonstrated in snapshoting the folding of cytochrome c back to 54% at 4.5 millisecond after completely mixing.
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Received: 2016-04-12
Accepted: 2016-08-09
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Corresponding Authors:
TAO Ye
E-mail: taoy@ihep.ac.cn
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