1. School of Environmental Studies and Key Laboratory of Biogeology and Environmental Geology of Chinese Ministry of Education, China University of Geosciences, Wuhan 430074, China 2. School of Material Science and Chemistry Engineering, China University of Geosciences, Wuhan 430074, China
Abstract：According to the principle that Zn(OH)+ produced by ZrOCl2 upon hydrolysis in water can react to form a fluorescent derivative with fructose, a method for the quantitative determination of fructose has been proposed. The fluorescence of the derivative enhanced at 408 nm in NH4Cl medium has a direct ratio with fructose in the linear range of 0.5-25 μg·mL-1. When reaction conditions such as a temperature of 70 ℃ and a time of 15 min are used, the detection limits is 0.071 mg·mL-1. The proposed method was also applied successfully to the determination of fruit, satisfaction with high accuracy and good reproduction.
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