Abstract：An orange-red [Ru(bpy)3]2+-DNA-Cu2+ composite film (bpy = 2,2′-bipyridine) was fabricated on an indium-tin (ITO) surface based on electrostatic interactions among [Ru(bpy)3]2+, DNA and Cu2+ by using self-standing cast methods. The photoinduced electron transfer (PET) properties of the resultant composite film mediated by DNA were studied by means of steady-state and time-resolved fluorescence spectroscopy, UV-visible absorption spectroscopy, fluorescence microscopic imaging and scan electron microscopy. The [Ru(bpy)3]2+-DNA-Cu2+ composite film with molar ratio of 10∶20∶1 shows an obvious absorption band (450 nm) and an intense emission peak (λem=595 nm), whose emission exhibits a single-exponential decay with τ=188.6 ns and is quenched by Cu2+via DNA-mediated PET mechanism, indicating that the quenching constant is 6.94×103 L·mol-1 and quenching rate constant is 3.80×1010 L·mol-1·s-1. The increasing molar ratio of Cu2+ in composite films (10-fold) leads to an 11 nm blue-shift of the emission peak, which is dramatically weakened by Cu2+via a static quenching mechanism. In addition, compared with the emission quenching of DNA-[Ru(bpy)2(tatp)]2+ (tatp=1,4,8,9-tetra-aza-triphenylene) tuned by Cu2+, which is present either in solutions or in composite films, Cu2+ only quenches the emission of [Ru(bpy)3]2+ bound to DNA via an electrostatic interaction mode in composite films.
Key words：Polypyridyl ruthenium(Ⅱ) complex;DNA;Copper(Ⅱ) ion;Composite film;Emission quenching;Photoinduced electron transfer
江臻燊, 陈敏健, 李 红* . DNA介导复合膜中[Ru(bpy)3]2+与Cu(Ⅱ)间光诱导电子转移 [J]. 光谱学与光谱分析, 2013, 33(09): 2332-2337.
JIANG Zhen-shen, CHEN Min-jian, LI Hong* . Photoinduced Electron Ttransfer between [Ru(bpy)3]2+ and Copper(Ⅱ) Ion in Composite Films Mediated by DNA . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(09): 2332-2337.
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