1. College of Chemistry and Chemical Industry, Anqing Teachers College, Anqing 246003, China 2. College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China
Abstract：The interaction between norfloxacin and bovine serum albumin，and the influence of Zinc(Ⅱ) on the system of norfloxacin and bovine serum albumin was studied under physiological condition by fluorescence method. It was shown that norfloxacin has a powerful ability to quench the BSA fluorescence via a nonradiative energy transfer mechanism. The fluorescence quenching data were analyzed according to Stern-Volmer equation and double-reciprocal equation, and the binding constant(K) and the binding sites(n) were obtained. In the system of binary complex of NFLX and BSA, K=6.80×105 and n=1.21. There is a strong combination between NFLX and BSA, which offers the condition for the serum protein to be deposited and transported in vivo. Besides, the combination between NFLX and BSA becomes stronger in the presence of Zinc(Ⅱ). According to Stern-Volmer equation and double-reciprocal equation, the concentration of Znic(Ⅱ) is denser, and the binding constant(K) and the binding sites(n) are bigger. By studying the binding interaction between Zinc(Ⅱ), norfloxacin and BSA, the mechanism of the interaction among norfloxacin, Zinc(Ⅱ) and protein in organism, is furtherly discussed.
Key words：Fluorescence spectrum;Norfloxacin;Zinc ions;Bovine serum albumin
[1] HUANG Pei-li，TIAN He-zhen(黄佩丽，田荷珍). Fundamental Elementary Chemistry(基础元素化学). Beijing: Beijing Normal University Press(北京: 北京师范大学出版社)，1994：346. [2] CAI Shao-hua，HUANG Kun-yao，ZHANG Yu-rong, et al(蔡少华，黄坤耀，张玉容, 等). Elemeotary Inorganic Chemistry(元素无机化学). Guangzhou: Zhongshan University Press(广州: 中山大学出版社)，1998. 212. [3] YANG Man-man，YANG Pin，ZHANG Li-wei(杨曼曼，杨 频，张立伟). Chinese Science Bulletin(科学通报)，1994，39(1)：31. [4] YAN Cheng-nong，SHANGGUANG Yun-feng，TONG Jin-qiang, et al(颜承农，上官云凤，童金强, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003，23(3)： 543. [5] Alain M，Michel B，Michel A. J. Chem. Edu.，1986，63(4)： 365. [6] ZHANG Yong，ZHANG Gui-zhu，WANG Yue-mei, et al(张 勇，张贵珠，王月梅, 等). Journal of Analytical Science(分析科学学报)，2000，16 (6)：447. [7] HUANG Shu-ping，YANG Man-man，CHEN Liang(黄淑萍，杨曼曼，陈 亮). Journal of Instrumental Analysis(分析测试学报)，1995，14(5)：57. [8] Shen L L，Pemat A G. Proceedings of National Academy of Science of the United States of America，1985，82：307.
