Study of Interaction of Ciprofloxacin Probe and Calf Thymus DNA Using Fluorescence Spectrometry
GAN Ning1, 2, GE Cong-xin1
1. College of Material Science and Chemical Engineering, Department of Chemistry, Ningbo University, Ningbo 315211, China 2. State Key Laboratory of Coordination Chemistry, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
Abstract：The interaction of ciprofloxacin and calf thymus DNA (ctDNA) was studied using UV spectrometry and fluorescence spectrometry. Ciprofloxacin exhibited a fluorescence excitation at 270 nm and a fluorescence emission at 420 nm in pH 7.0 phosphate buffer solutions due to the groove binding of ciprofloxacin on calf thymus ctDNA. The binding constant was 2.64×104 mol-1·L(25 ℃). Based on the fluorescence quenching, a novel method for sensitive determination of calf thymus ctDNA concentration ranging from 80 nmol·L-1 to 45 μmol·L-1 was developed. The relative standard deviation for eleven detections of 25 μmol·L-1 ctDNA was 4.2%.
Key words：Ciprofloxacin;Fluorescence quenching;Groove binding;Calf thymus DNA
干宁1,2,葛从辛1. 环丙氟沙星荧光探针对小牛胸腺DNA相互作用的研究[J]. 光谱学与光谱分析, 2006, 26(12): 2276-2279.
GAN Ning1, 2, GE Cong-xin1. Study of Interaction of Ciprofloxacin Probe and Calf Thymus DNA Using Fluorescence Spectrometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2276-2279.
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