Determination of Cephradine Capsules Content Based on Fluorescence Quenching Method of FOS-μSIA-LOV
QIU Xin1, LI Xin-xia2, Arkin IBURAIM2, Gulina KUERBAN3, ZHANG Li-hua1, LI Yan1*
1. Department of Pharmacy, Maternal and Child Health Care of Zaozhuang, Zaozhuang 277100, China
2. Central Laboratory of Xinjiang Medical University, Urumqi 830011, China
3. Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China
Abstract：The content of cephradine capsules was determined by a system of FOS-μSIA-LOV(fiber optic sensor-micro sequential injection-valve laboratory), based on the principle of cefradine quenching fluorescein in methanol solvent, optimize experimental conditions. The order of inhalation was anhydrous methanol, cefradine, fluorescein and anhydrous methanol; the carrier liquid suction volume was 500 μL; cefradine inhalation volume was 100 μL. Cefradine quenched fluorescein fluorescence intensity and concentration in 0.05～0.5 mg·mL-1 linear relationship as ΔF=812.6+284.41, r=0.999 5, precision RSD was 4.4%, the recovery was 101%～103%. FOS-SIA-LOV and personalized drug testing and programming resulted in automated on-line process analysis with the good reproducibility, less reagent consumption, time and labor saving.
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