1. Key Laboratory for Bio-Chemistry and Organic Chemistry, Zhengzhou University, Zhengzhou 450052, China 2. Anyang Teachers College, Anyang 455002, China 3. Key Laboratory for Bio-organic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China
摘要： 以溴化乙锭(EB)为荧光探针，研究了7-羟基黄酮及磷酰化7-羟基黄酮与DNA的弱相互作用。实验结果表明，两种化合物与DNA间均存在弱相互作用，但与7-羟基黄酮相比，磷酰化7-羟基黄酮对DNA更具亲和力。随着温度的升高，7-羟基黄酮及磷酰化7-羟基黄酮对DNA-EB体系的荧光猝灭常数降低，两种化合物均可与DNA形成复合物，此猝灭过程为静态猝灭。根据Stern-Volmer方程和Scatchard方程，常温下7-羟基黄酮及磷酰化7-羟基黄酮对DNA-EB体系的荧光猝灭常数和它们与DNA的固有的结合常数分别为：Kq1=601 L·mol-1,Kq2=1 381 L·mol-1;K1=2.07×104 L ·mol-1,K2=3.19×104 L·mol-1。
关键词：EB探针;DNA;7-羟基黄酮;磷酰化7-羟基黄酮;荧光猝灭;弱相互作用
Abstract：The non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA was studied using ethidium bromide(EB)as a probe. The result showed that both 7-hydroxy flavone and its phosphate could form non-covalent complexes, but the phosphorylated flavonoid showed higher binding affinity with DNA than 7-hydroflavone did. Experiments demonstrated that the higher the temperature, the lower the slop of quenching curve of DNA-EB in the presence of different amounts of 7-hydroxy flavone and its phosphate. It was confirmed that the combinations of DNA with 7-hydroxy flavone and its phosphate were a single static quenching process. According to the Stern-Volmer equation and Scatchard equation the quenching constants and the intrinsic binding constants of 7-hydroxy flavone and its phosphate were measured respectively, they were Kq1= 601 L·mol-1,Kq2=1 381 L·mol-1;K1=2.07×104 L·mol-1,K2=3.19×104 L·mol-1 respectively.
郭玉华1,陈晓岚1,张 婷1,屈凌波1, 2*,赵玉芬1,3,郁有祝1 . 7-羟基黄酮及磷酰化7-羟基黄酮与DNA的弱相互作用荧光法研究[J]. 光谱学与光谱分析, 2006, 26(03): 475-479.
GUO Yu-hua1,CHEN Xiao-lan1,ZHANG Ting1,QU Ling-bo1, 2*,ZHAO Yu-fen1, 3,YU You-zhu1 . Study on the Non-Covalent Interaction of 7-Hydroxy Flavone and Its Phosphate with DNA by Fluorescence Method. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(03): 475-479.
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