The Reaction Mechanism between Cefoperazone and Human Serum Albumin
LIU Luo-sheng1，ZHANG Yu-yi2，WANG Xing-po1
1. School of Chemistry and Chemical Engineering, Shandong University, Ji’nan 250100, China 2. School of Medicine, Shandong University, Ji’nan 250012, China
Abstract：The reaction mechanism between cefoperazone and human serum albumin(HSA) and the affinity between cefoperazone and β-lactamase were investigated by spectrometry and spectrofluorimetry. The interaction dissociation constants of human serum albumin and cefoperazone have been determined from a double reciprocal Lineweaver-Burk plot. The binding distance and the transfer efficiency between cefoperazone and HSA were also obtained according to the theory of Frster’ non-radiation energy transfer. The result suggested that the main binding force between cefoperazone and HSA is electrostatic force interaction. The high β-lactamase stability of cefoperazone may be correlative with its molecular structure. The antibiotic activity and valency connect with transfer efficiency and dissociation constant. The effect of cefoperazone on the conformation of HSA was also analyzed using synchronous fluorescence spectrometry.
刘洛生1，张虞毅2，王兴坡1. 头孢哌酮与人血清白蛋白相互作用机制[J]. 光谱学与光谱分析, 2005, 25(09): 1490-1492.
LIU Luo-sheng1，ZHANG Yu-yi2，WANG Xing-po1. The Reaction Mechanism between Cefoperazone and Human Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1490-1492.
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