Immunoassay of Estradiol by an Array Evanescent Wave Fluorescent Biosensor
SHAN Di-di1, 3, WEN Xiao-gang2*, LIU Lan-hua1, ZHOU Xiao-hong1*, HE Miao1, SHI Han-chang1
1. State Key Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China
2. Jiaxing University, Nanhu College, Jiaxing 314001, China
3. Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China
Abstract：In recent years, environmental endocrine disruptors have attracted more and more attention. Traditional detection methods are time-consuming, complex and expensive, which means the development of fast response, high sensitivity and low cost detection method is of vital importance. Herein we described a planar waveguide-based array evanescent wave fluorescent biosensor. Based on the simulation and optimization of the optical parameters of the system, it allows the measurements of up to twenty-four sensing spots simultaneously, rapidly and highly sensitive. By using the indirect competitive immunoassay, the detection conditions of the array evanescent wave fluorescent biosensor were optimized and established for the environmental endocrine disruptor of estradiol. The experimental results show that the system has the highest sensitivity at 61.8°. The linear response ranged from 0.08~2.52 μg·L-1 has the detection limit of 0.05 μg·L-1 and the 50% inhibition concentration (IC50) of 0.46 μg·L-1. One test cycle was completed within 20 min. The recoveries of estradiol in all sorts of wastewater effluents ranged from 84% to 120% has relative standard deviation values of less than 15%, confirming the application potential in the measurement of estradiol in reality.
[1] LI Feng-ling, JIANG Yan-hua, YAO Lin(李风铃, 江艳华, 姚 琳). Chinese Fishery Quality and Standards(中国渔业质量与标准)，2013, 3(1)：44.
[2] Filby A L, Paull G C, Searle F, et al. Environmental Science & Technolog, 2012, 46(6): 3472.
[3] Butle D, Guilbault G G. Sensors and Actuators B: Chemical, 2006, 113：692.
[4] Kumar R, Malik A K, Kabir A, et al. Journal of Chromatography A, 2014, 1359：16.
[5] Hao X, Zhou X, Zhang Y, et al. Sensors and Actuators B: Chemical, 2014, 204：682.
[6] Liu L, Zhou X, Shi H. Biosensors and Bioelectronics, 2015, 72：300.
[7] Liu L, Zhou X, Lu M. Sensors and Actuators B: Chemical, 2017, 240：107.
[8] Chang C C, Huang S D. Analytica Chimica Acta, 2010, 662：39.
[9] CAO Li-bo(曹立波). Heilongjiang Textile(黑龙江纺织), 2011, 4：14.
[10] FU Yin-jie, GAO Yan-zheng, DONG Chang-xun, et al((付银杰, 高彦征, 董长勋, 等). Journal of Agro-Environment Science(农业环境科学学报), 2012, 31(11)：2296.
[11] MA Yan, CAO Wei, GONG Pei-xue, et al(马 艳, 曹 伟, 公丕学, 等). Journal of Instrumental Analysis(分析测试学报), 2010, 29(4)：390.
[12] NIU Shu-cao, Lü Zhen-zhen, LIU Jin-chuan, et al(牛书操, 吕珍珍, 刘金钏, 等). Journal of Instrumental Analysis(分析测试学报), 2014, 33(7)：835.
[13] Zhou X, Song B, Shi H, et al. Sensors and Actuators B: Chemical, 2014, 198：150.
