Abstract：The adsorption of p-aminothiophenol (PATP) molecule on the gold electrode was investigated. The result suggests that PATP was adsorbed perpendicularly onto the substrate. By immobilizing goat anti-mouse IgG on a glass slide modified by special organic molecules, the antibody/antigen/reporter-labeled immunogold nanoparticle sandwich assay was build for surface-enhanced Raman spectroscopy(SERS) measurements. The p-aminothiophenol(PATP) was used as SERS-reporter molecule. Thus the authors can identify the single type biospecific species by SERS signal of the reporter molecules which are coimmobilized with antibody molecules in gold colloids.
[1] Ni J, Lipert R J, Dawson G B, et al. Anal. Chem., 1999, 71: 4903. [2] QIU Li-qun, GU Ren-ao(仇立群，顾仁敖). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(5): 547. [3] Michota A, Bukowska J. J. Raman Spectrosc., 2003, 34: 21. [4] Sokolow K, Byramova N E, Mochalova L V, et al. Appl. Spectrosc., 1993, 47: 535. [5] Morjani H, Rjou, J F, Nabiev, I R, et al. Manfait Cancer Res., 1993, 53: 4784. [6] Nabiev I R, Morjani H, Manfait M. Eur. Biophys. J., 1991, 19: 311. [7] Picorel R, Holt R E, Cotton T M, et al. J. Biol. Chem., 1988, 263: 4374. [8] ZHU Zi-hua, ZHU Tao, LIU Zhong-fan(朱梓华，朱 涛，刘忠范). Acta Phys. Chim. Sin.(物理化学学报), 1999, 15(11): 996. [9] LI Xiao-wei, ZHENG Jun-wei, ZHOU Yao-guo, et al(李晓伟，郑军伟，周耀国，等). Chinese Journal of Analytical Chemistry(分析化学), 2003，31(11): 1333. [10] Osawa M，Matsuda N，Yoshll K，et al. J. Phys. Chem. B, 1994, 98: 12702. [11] Zeman E J, Schatyz G C. J. Phys. Chem., 1978, 91: 634. [12] Grubisha D S, Lipert R J, Park H Y, et al. Anal. Chem., 2003, 75: 5936.
