Study of Regeneration Based on SERS Labelled Immunoassay
GE Ming1, 2, YAO Jian-lin1, SUN Ru1, GU Ren-ao1*
1. College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China 2. College of Chemistry and Chemical Engineering, Nantong University, Nantong 226001, China
Abstract：Labelled immunoassay by surface enhanced Raman scattering(SERS) has great research and application value. It combines SERS which has the high sensitivity and high selectivity with specific adsorption in immunology. The present paper mainly studies the regeneration about SERS labelled immunoassay, striving to develop the recycling value of it. The authors used glycine-HCl eluent for the sandwich structure including solid matrix antibody, antigen and labelled immuno-gold colloids, then the authors had got expected result. The complex of antibody and antigen would be separated by changing the pH scale. It could elute the most antigen and the labelled immuno-gold colloids. Also the authors could assemble it again and distinguish the characteristic SERS spectrum of the reporter molecules. Under this condition, we researched the stability and reusing of this technology. The authors found that it has better stability and it retained activity after 10 recycles of applications.
葛 明1, 2，姚建林1，孙 如1，顾仁敖1* . 基于SERS标记免疫检测的再生性研究 [J]. 光谱学与光谱分析, 2010, 30(07): 1785-1788.
GE Ming1, 2, YAO Jian-lin1, SUN Ru1, GU Ren-ao1* . Study of Regeneration Based on SERS Labelled Immunoassay . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(07): 1785-1788.
[1] Fleischmann M, Hendra P J, McQuillan A J. Chem. Phys. Lett., 1974, 26: 163. [2] Jeanmaire D J, Van Duyne R P. J. Electroanal. Chem., 1977, 84: 1. [3] Tian Z Q. J. Raman Spectrosc., 2005, 36: 466. [4] GE Ming，CUI Yan，GU Ren-ao(葛 明，崔 颜，顾仁敖). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(1): 110. [5] DONG Zhi-wei，WANG Yan(董志伟，王 琰). Antibody Engineering(抗体工程). Beijing: Beijing Medical University Press(北京: 北京医科大学出版社)，2002. 209. [6] LI You-ping(李幼平). Principle and Choice of Medical Experimental Technology(医学实验技术的原理与选择). Beijing: People’s Medical Publishing House(北京: 人民卫生出版社)，2008. 370. [7] Miguel A G, Rosa P, Maquieira A. Trends in Analytical Chemistry, 1999, 18: 204. [8] Frens G. Nature Phy. Sci., 1973, 241: 20.
