1. Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Nanjing 210093, China 2. Department of Biochemistry, Suzhou Teachers' College, Suzhou 234000, China
Abstract：In order to understand the mechanism of the interaction of sulfate polysaccharides and small molecules at molecular level, the structure of pachymaran was decorated by Wolfrom, and sulfation pachymaran(SP) was prepared. The maximum binding number(N=54) and the binding equilibrium constant(K=1.563×106) of the sulfation pachymaran and methylene blue (MB) were obtained by the exoterica model. The influence of the molar ratio of MB/SP，ethanol，hydroxypropyl-β-cyclodextrin，Triton X-100 and NaCl on the spectra of MB-SP complex was investigated. The mechanism of the color changes of methylene blue and sulfation pachymaran reaction system has been discussed. The authors draw the conclusion that the color changes result mainly from the hydrophobic interaction between methylene blue molecules binding on sulfation pachymaran on the base of the electrostatic interaction of methylene blue and sulfation pachymaran.
Key words：Sulfation pachymaran;Methylene blue;Spectrometry;Maximum binding number
高贵珍1, 2，焦庆才1，丁一磊1，陈 雷1 . 亚甲蓝与硫酸化茯苓多糖相互作用机理的研究[J]. 光谱学与光谱分析, 2005, 25(04): 608-612.
GAO Gui-zhen1, 2, JIAO Qing-cai1, DING Yi-lei1，CHEN Lei1 . Study on the Mechanism of the Interaction of Methylene Blue and Sulfation Pachymaran . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(04): 608-612.
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