Abstract:The instant effect of temperature on the absorption spectra of the hemoglobin in single living intact red blood cells was investigated, by employing a highly sensitive fast multi-channel micro-spectrophotometer system to perform non-invasive, in situ, real time measurements on the cells. It was found that both the heights and position of the specific peaks in the absorption spectra of intercellular hemoglobin were changed with temperature, indicating that the oxygen carrying capacity of red blood cells varies with temperature. The correlations of the structure and concentration as well as the function of hemoglobin, and the molecular mechanism were also discussed.
姚成灿1,李校坤1,黄耀熊2* . 温度对单个活态人红细胞携氧能力的即时影响[J]. 光谱学与光谱分析, 2005, 25(04): 613-616.
YAO Cheng-can1, LI Xiao-kun1, HUANG Yao-xiong2* . Instant Effect of Temperature on the Oxygen Carrying Capacity of Single Living Intact Red Blood Cell . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(04): 613-616.
[1] YAO Cheng-can, HUANG Yao-xiong, LI Xiao-kun et al(姚成灿, 黄耀熊, 李校坤等). Chinese Science Bull.(科学通报), 2003, 48(13): 1342. [2] LI Jing, HUANG Yao-xiong(李 晶, 黄耀熊). Acta Biophysica Sinica(生物物理学报), 2002, 18(3): 350. [3] JI Tao, HUANG Yao-xiong(籍 涛, 黄耀熊). Acta Biophysica Sinica(生物物理学报), 2001, 17(2): 599. [4] Rachmilewitz E A. Semin. Hematol., 1974, 11: 441. [5] Farahani K, Saxton R E, Yoon H C et al. Magnetic Resonance Imaging, 1999, 17: 1489. [6] Steel B, Bilek M M, Dos Remedios C G et al. Eur. Biophys. J., 2004, 33(2): 117. [7] Ivanov I T. J. of Thermal Biology, 1999, 24: 143. [8] Frans L A W, Bregt R S, Yvonne A M G et al. Blood, 2003, 101: 747. [9] Gervais P, de Maraón I M, Evrard C et al. J. of Biotech., 2003, 102: 269.