Determination of Chinese Traditional Medicine Activity Eliminating Superoxide Anion Radical by Kinetics Spectrophotometry
TIAN Yi-ling1, CHEN Guan-hua2, CUI tong1
1. College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China 2. College of Life Science, Agricultural University of Hebei, Baoding 071001, China
Abstract：A new method to determine Chinese traditional medicine activity eliminating superoxide anion radical by kinetic spectrophotometry was developed. It is shown that the optimum determination condition may be obtained when wavelength is 520 nm, the concentration of enzyme is 4×10-3 μg·mL-1, the reaction time is between 2-7 min. Vitamin C activity eliminating superoxide anion radical was determined under the condition, and the results were identical with literature. The method is used to determine IC50 of Cortex Magnoliae Officinalis and Cortex Eucommia, and they are 7.580 and 323.800 mg·L-1 respectively.
Key words：Kinetics spectrophotometry;Superoxide anion radical;Extractive Chinese traditional medicine
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