Study on the Effect of High Hydrostatic Pressure Treatment on the Secondary Structure of Mushroom Polyphenoloxidase by SRCD and FTIR
YI Jian-yong, DONG Peng, WANG Yong-tao, JIANG Bin, LIAO Xiao-jun, HU Xiao-song, ZHANG Yan*
National Engineering Research Centre for Fruit and Vegetable Processing; College of Food Science and Nutritional Engineering, China Agricultural University;Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Beijing 100083, China
Abstract：The secondary structure of the mushroom polyphenoloxidase treated by the high hydrostatic pressure (HHP) was analyzed by the synchrotron radiation circular dichroism (SRCD) and Fourier transform infrared spectroscopy (FTIR). The α-helix content of mushroom PPO was decreased after HHP treatment, which indicated that the secondary structure of PPO was changed. There was a discrepancy of the result of the secondary structure content between untreated or HHP-treated mushroom PPO analyzed by SRCD and FTIR spectra, and this discrepancy may be due to the different determination temperature, the concentration of the PPO solution and the spectra analysis method etc. The fluorescence spectra showed that the fluorescence intensity of the mushroom PPO was decreased after HHP treatment, and a red shift was observed after HHP treatment, which indicated that the tertiary structure of the enzyme molecule has been modified.
易建勇，董 鹏，王永涛， 姜 斌，廖小军，胡小松，张 燕* . 应用SRCD和FTIR分析超高压处理对蘑菇多酚氧化酶二级结构的影响[J]. 光谱学与光谱分析, 2012, 32(02): 317-323.
YI Jian-yong, DONG Peng, WANG Yong-tao, JIANG Bin, LIAO Xiao-jun, HU Xiao-song, ZHANG Yan* . Study on the Effect of High Hydrostatic Pressure Treatment on the Secondary Structure of Mushroom Polyphenoloxidase by SRCD and FTIR. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(02): 317-323.
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