Abstract：In the present study, the feasibility of using wavelet analysis to extract the eigen spectra from the absorption spectra of phytoplankton for species detection was investigated. Thirteen absorption spectra taken from single species cultures, representing four divisions (Dinophyta, Bacillariophyta, Haptophyta, and Chlorophyta), six genus (Gymnodinium, Prorocentrum, Skeletonema, Guinardia, Phaeocystis,and Prasinophyte) and seven species(Karenia mikimotoi, Karenia brevis, Prorocentrum minimum, Skeletonema costatuma, Guinardia delicatula, Phaeocystis globosa, and Pyramimonas parkeae), were used. First, the 1D wavelet analysis with five levels was applied to the thirteen absorption spectra, so each spectrum was decomposed with 5 levels. The 5th level component of low frequency corresponds to the background without information for species detection, and 1st and 2nd level component of high frequency is the random noise. The other levels (3rd to 5th) of high frequency are the useful information, and the sum of levels (3rd to 5th) of high frequency was retained as the eigen spectra for species detection. Second, the clustering analysis was used to the eigen spectra for examining the performance of the wavelet analysis in extracting species information. The clustering results were compared with the known species class information, and the results show that the 13 absorption spectra are correctly classified at the level of division, genus and species. This means that the wavelet analysis has good performance in extracting the eigen spectra for species detection. However, the above results were obtained with only limited species, and the more species data are required to identify the extensive validity of the conclusion.
Key words：Wavelet analysis;Absorption spectra of phytoplankton;Species detection;Harmful algal bloom
张亭禄，杜祥之，徐青娜，邱国强. 一维小波分析在浮游植物吸收光谱有害赤潮藻类检测中的应用[J]. 光谱学与光谱分析, 2009, 29(10): 2743-2747.
ZHANG Ting-lu, DU Xiang-zhi, XU Qing-na, QIU Guo-qiang. Application of 1D Wavelet Analysis in Detecting Species of Harmful Algae Blooms with Absorption Spectra of Phytoplankton . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(10): 2743-2747.
[1] Kishino M, Okami N, Takahashi M, et al. Bull. Mar. Sci., 2004, 37: 634. [2] Lerebourg C J, Pilgrim D A, Ludbrook G D, et al. J. Opt., 2002, 4: 56. [3] Zaneveld J R V, Moore C, Barnard A H, et al. Ocean. Optics, 2004, ⅩⅤⅡ: 44. [4] Lee Z P, Carder L. Remote Sensing Environment, 2004, 89: 361. [5] Cullen J J, Ciotti A M, Davis R F, et al. Limnology and Oceanography, 1997, 42(5): 1223. [6] Schofield O, Grzymski K, Bissett W P, et al. J. Phycol., 1999, 35: 1477. [7] Babin M, Cullen J J, Roesler C, et al. Oceanography, 2005, 18(2): 210. [8] Hooks C E, Bidigare R R, Keller M D, et al. J. Phycology, 1988, 24(4): 571. [9] Bidigare R R, Ondrusek M E, Morrow J H, et al. Ocean. Optics, 1990, Ⅹ: 290. [10] Hoepffner N, Sathyendranath S. J. Geophys. Res., 1993, 98: 22789. [11] Stuart V, Sathyendranath V S, Platt T, et al. J. Plankton Res., 1998, 20: 187. [12] Ciotti A M, Lewis M R, Cullen J J. Limnology and Oceanography, 2002, 47: 404. [13] Bricaud A, Claustre H, Ras J, et al. J. Geophys. Res., 2004，109: C11010. [14] Staehr P A, Cullen J J. J. Plankton Res., 2003, 25: 1237. [15] Johnsen G O, Samset L G, Sakshang E. Mar. Ecol. Prog. Ser., 1994, 105: 149. [16] Millie D F, Schofield O M, Kirkpatrixk G J, et al. Limnology and Oceanography, 1997, 42: 1240. [17] Kirkpatrick G J, Millie D F, Moline M A, et al. Limnology and Oceanography, 2000, 45: 467. [18] YANG Sheng-bo, YU Chun-mei(杨胜波，于春梅). Chinese Journal of Scientific Instrument(仪器仪表学报)，2002, 23(3): 179. [19] LU Jia-hui, ZHANG Yi-bo, ZHANG Zhuo-yong, et al(逯家辉，张益波，张卓勇，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2008，28(6)：1264. [20] Astorecal R, Rousseaul V, Ruddick K, et al. Ocean. Optics, 2004, ⅩⅤⅡ:68. [21] CHENG Zhen-xing(程振兴). Logarithms and Application of Wavelet Analysis(小波分析算法和应用). Xi’an: Xi’an Jiaotong University Press(西安: 西安交通大学出版社), 1998.
